Results 21 to 30 of about 224,524 (254)

SPI-7: Salmonella’s Vi-Encoding Pathogenicity Island

open access: yesJournal of Infection in Developing Countries, 2008
Salmonella Pathogenicity Island-7 (SPI-7) is a large, mosaic, genetic island, found in several serovars of Salmonella enterica subsp. enterica associated with systemic disease.
Helena M. B. Seth-Smith
doaj   +1 more source

Sanger sequencing [PDF]

open access: yesThe FEBS Journal, 2017
This poster beautifully illustrates the process and importance of Sanger sequencing. Developed by Diego Estrada-Rivadeneyra, it won one of the three 50th anniversary science communication competition prizes.
openaire   +3 more sources

Optimization in Sanger sequencing

open access: yesComputers & Operations Research, 2019
The main objective of this paper is to solve the optimization problem that is associated with the classification of DNA samples in PCR plates for Sanger sequencing. To achieve this goal, we design an integer linear programming model. Given that the real instances involve the classification of thousands of samples and the linear model can only be solved
Luisa Carpente   +3 more
openaire   +5 more sources

Characterization of gene mutations and copy number changes in acute myeloid leukemia using a rapid target enrichment protocol

open access: yesHaematologica, 2015
Prognostic stratification is critical for making therapeutic decisions and maximizing survival of patients with acute myeloid leukemia. Advances in the genomics of acute myeloid leukemia have identified several recurrent gene mutations whose prognostic ...
Niccolò Bolli   +17 more
doaj   +1 more source

Whole-exome sequencing improves genetic testing accuracy in pulmonary artery hypertension

open access: yesPulmonary Circulation, 2018
Sanger sequencing, the traditional “gold standard” for mutation detection, has been wildly used in genetic testing of pulmonary artery hypertension (PAH). However, with the advent of whole-exome sequencing (WES), few studies have compared the accuracy of
Xiaofang Zeng   +9 more
doaj   +1 more source

BRAF V600E Mutation Test in Fine Needle Aspiration (FNA) Specimens of Thyroid Nodules by Competitive Allele-Specific TaqMan PCR (castPCR)

open access: yesIndonesian Journal of Cancer, 2023
Background: BRAF V600E mutation has been confirmed as a significant molecular indicator for the diagnosis and prognosis of papillary thyroid carcinoma (PTC).
Adhitya Bayu Perdana   +3 more
doaj   +1 more source

Identification of rare thalassemia variants using third-generation sequencing

open access: yesFrontiers in Genetics, 2023
Routine PCR, Sanger sequencing, and specially designed GAP-PCR are often used in the genetic analysis of thalassemia, but all these methods have limitations.
Qin Liu   +11 more
doaj   +1 more source

Sanger Sequencing v1 [PDF]

open access: yes, 2018
For sequence by dideoxy-method using the ABI3130 platform (Applied Biosystems, USA) and specific primers.
Lívia Sacchetto, Izabela M Rezende
openaire   +1 more source

Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods

open access: yesScientific Reports, 2022
Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely.
Sujata Balasubramanian   +16 more
doaj   +1 more source

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