Results 11 to 20 of about 224,524 (254)
A Sequel to Sanger: amplicon sequencing that scales [PDF]
BMC Genomics, 2018 Background Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing.
Paul D. N. Hebert +10 more
doaj +3 more sources
From Sanger sequencing to genome databases and beyond [PDF]
BioTechniques, 2019 We look at how next-generation sequencing has advanced research across different disease fields, and the growing importance of open access genomic databases. [Graphic: see text]
Jenny Straiton +3 more
doaj +2 more sources
SangeR: the high-throughput Sanger sequencing analysis pipeline [PDF]
Bioinformatics Advances, 2022 Abstract Summary In the era of next generation sequencing and beyond, the Sanger technique is still widely used for variant verification of inconclusive or ambiguous high-throughput sequencing results or as a low-cost molecular genetical analysis tool for single targets in many fields of study.
Kai Schmid +7 more
openaire +6 more sources
cDNA sequencing increases the molecular diagnostic yield in Chediak-Higashi syndrome
Frontiers in Genetics, 2023 Introduction: Chediak-Higashi syndrome (CHS) is rare autosomal recessive disorder caused by bi-allelic variants in the Lysosomal Trafficking Regulator (LYST) gene.
Chulaluk Kuptanon +17 more
doaj +1 more source
Detection of BRAF V600E in Fine-Needle Aspiration Samples of Thyroid Nodules by Droplet Digital PCR
International Journal of Endocrinology, 2022 Background. BRAF exon 15 p.V600E (BRAF V600E) mutation has been established as an important molecular marker for papillary thyroid carcinoma diagnosis by ultrasound-guided fine-needle aspiration biopsy (FNAB).
Sang-Yu Lu +14 more
doaj +1 more source
Mutation Test in Korean Patients with Colorectal Carcinomas: A Methodological Comparison between Sanger Sequencing and a Real-Time PCR-Based Assay [PDF]
Journal of Pathology and Translational Medicine, 2017 Background Mutations in the KRAS gene have been identified in approximately 50% of colorectal cancers (CRCs). KRAS mutations are well established biomarkers in anti–epidermal growth factor receptor therapy.
Sung Hak Lee +6 more
doaj +1 more source
Diagnostics, 2022 Monoclonal rearrangements of immunoglobulin (Ig) genes and T-cell receptor (TCR) genes are used for minimal measurable disease in acute lymphoblastic leukemia (ALL).
Karin Paulsen +4 more
doaj +1 more source
BMC Medical Genomics, 2023 Background This study aimed to compare the performance of Sanger-based SARS-CoV-2 spike gene sequencing and Next Generation Sequencing (NGS)-based full-genome sequencing for variant identification in saliva samples with low viral titer. Methods Using 241
Ko Ko +13 more
doaj +1 more source
A Python script to merge Sanger sequences [PDF]
PeerJ, 2020 Abstract Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to merge multiple overlapping Sanger sequences. Our results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run.
Cen Chen +8 more
openaire +3 more sources
Diagnostics, 2023 Variants of concern (VOCs) of SARS-CoV-2 are viral strains that have mutations associated with increased transmissibility and/or increased virulence, and their main mutations are in the receptor binding domain (RBD) region of the viral spike.
Grazielle Motta Rodrigues +5 more
doaj +1 more source