International Journal of Infectious Diseases, 2020 Background: The clinical utilisation of deep sequencing in HIV treatment has been hindered due to its unknown correlation with standard Sanger genotyping and the undetermined value of minority drug resistance mutation (DRM) detection.
Ting-Shu Wu +2 more
exaly +3 more sources
Panel-based next-generation sequencing with copy number variant detection pipeline could increase the detection rate of hereditary colorectal cancer syndrome compared with Sanger sequencing [PDF]
Hereditary Cancer in Clinical PracticeBackground Hereditary colorectal cancer syndromes, such as Lynch syndrome and familial adenomatous polyposis, arise from pathogenic/likely pathogenic (P/LP) germline variants in DNA mismatch repair or tumor suppressor genes. Traditional Sanger sequencing
Joonsang Yu +8 more
doaj +2 more sources
A 3D-printed Sanger sequencing activity addresses student misconceptions [PDF]
Journal of Microbiology & Biology EducationThe process of Sanger sequencing can be a challenging and unintuitive concept for students to master. In order to improve student learning, we developed a hands-on Sanger sequencing activity using 3D-printed models to incorporate tactile learning.
Phillip E. Brown, Stefanie H. Chen
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A Comparison of Sanger Sequencing and Amplicon-Based Next Generation Sequencing Approaches for the Detection of HIV-1 Drug Resistance Mutations [PDF]
VirusesBackground: Next-generation sequencing (NGS) kits are needed to finalise the transition from Sanger sequencing to NGS in HIV-1 genotypic drug resistance testing. Materials and Methods: We compared a homemade NGS amplicon-based protocol and the AD4SEQ HIV-
Camilla Biba +7 more
doaj +2 more sources
Comparative Analysis of Metagenomic Next-Generation Sequencing, Sanger Sequencing, and Conventional Culture for Detecting Common Pathogens Causing Lower Respiratory Tract Infections in Clinical Samples [PDF]
MicroorganismsMetagenomic next-generation sequencing (mNGS) has emerged as a revolutionary tool for infectious disease diagnostics. The necessity of mNGS in real-world clinical practice for common Lower Respiratory Tract Infections (LRTI) needs further evaluation.
Qiaolian Yi +7 more
doaj +2 more sources
Confirmation of Exome Sequencing Results Using Sanger Sequencing—Considerations in a Low‐Resource Setting [PDF]
Molecular Genetics & Genomic MedicineBackground Exome sequencing (ES) is now widely accepted as an appropriate first‐tier diagnostic test for developmental disorders (DD). International guidelines recommend that in diagnostic settings, ES findings be validated with an orthogonal method ...
Nadja Louw +10 more
doaj +2 more sources
Developmental Validation for Sanger Sequencing of HV1 and HV2 in Mitochondrial DNA
Forensic Science International: Reports, 2020 The method of Sanger sequencing of mitochondrial DNA (mtDNA) for forensic purposes has been validated previously. However, to our knowledge, there are few validation reports of Sanger sequencing of mtDNA that satisfy the latest SWGDAM Validation ...
Koji Fujii +2 more
exaly +3 more sources
Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Punyasloke Bhadury, Anwesha Ghosh
exaly +3 more sources
Newborn Genetic Screening—Still a Role for Sanger Sequencing in the Era of NGS [PDF]
International Journal of Neonatal Screening, 2023 Asbjorg Stray-Pedersen +2 more
exaly +2 more sources
NGS in the clinical microbiology settings
Frontiers in Cellular and Infection Microbiology, 2022 We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients.
Milena Pitashny +8 more
doaj +1 more source