spaMGCN: a graph convolutional network with autoencoder for spatial domain identification using multi-scale adaptation. [PDF]
Zhang T +6 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Portuguese version of the citizenship fatigue scale: adaptation and evidence of validity. [PDF]
Neves PC, Andrade C, Alves L.
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
French Version of the User Mobile Application Rating Scale: Adaptation and Validation Study. [PDF]
Saliasi I +12 more
europepmc +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Forgiveness in Poland: Rye Forgiveness Scale Adaptation and Its Longitudinal Impact on Mental Health in HIV-Positive Individuals. [PDF]
Skalski-Bednarz SB +2 more
europepmc +1 more source
Spanish Version of the Family Health Behavior Scale: Adaptation and Validation. [PDF]
Lanzarote-Fernández MD +5 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Measuring the Nursing Work Environment during Public Health Emergencies: Scale Adaptation and Validation. [PDF]
Wang X +9 more
europepmc +1 more source

