Results 131 to 140 of about 1,188,974 (311)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
The medial axis transform is a well-known tool for shape recognition. Instead of the object contour, it equivalently describes a binary object in terms of a skeleton containing all centres of maximal inscribed discs. While this shape descriptor is useful for many applications, it is also sensitive to noise: Small boundary perturbations can result in ...
Julia Gierke, Pascal Peter
openaire +2 more sources
Safe operating space for humanity at a regional scale
Ecology and Society, 2018 The planetary boundaries framework defined safe limits to human impacts on essential Earth-system processes. Subsequent assessments concluded that impacts exceed most delineated boundaries.
John F. McLaughlin
doaj +1 more source
Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish. [PDF]
iScience, 2022 Teranikar T +9 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Isophotes, Scale Space, and Invariants in Lung CT for COPD Diagnosis. [PDF]
Radiol Artif Intell, 2022 Vannier MW.
europepmc +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
, 2021 The ability to handle large scale variations is crucial for many real world visual tasks. A straightforward approach for handling scale in a deep network is to process an image at several scales simultaneously in a set of scale channels. Scale invariance
Jansson, Ylva,, Lindeberg, Tony,
core
Spectrum decomposition in Gaussian scale space for uneven illumination image binarization. [PDF]
PLoS One, 2021 Long J, Yan Z, Chen H, Song X.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source