Results 41 to 50 of about 1,477,560 (316)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Topical issues related to reference standards in the sphere of circulation of biological products

Биопрепараты: Профилактика, диагностика, лечение, 2018
Pharmacopoeial analytical methods involving reference standards (RS) are used for manufacture and quality control of medicinal products, including biologicals, these RS should now to be called pharmacopoeal.
R. A. Volkova, O. V. Fadeikina, V. I. Klimov, E. I. Sakanyan, Yu. V. Olefir, V. A. Merkulov, A. A. Movsesyants, V. P. Bondarev, I. V. Borisevich, D. V. Shvedov   +9 more
doaj  

A METHODOLOGICAL APPROACH TO DESIGNING EXPERIMENTS WHEN DEALING WITH IDENTIFICATION TESTS FOR MEDICINAL PRODUCT COMPONENTS (AS ILLUSTRATED BY ASCORBIC ACID)

Регуляторные исследования и экспертиза лекарственных средств, 2018
The need for identification  testing of active substances or excipients in multi-component medicinal products,  including the use of qualitative tests, calls for research substantiating the choice of tests and test conditions  with due regard to ...
Yu. B. Purim, M. L. Kruglyakova, L. N. Bulanova, M. V. Agapkina, L. N. Stronova, T. N. Bokovikova, E. P. Gernikova   +6 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Monoclonal antibodies: Development of universal (platform) methods for the assessment of high and low-molecular weight impurities

Регуляторные исследования и экспертиза лекарственных средств
INTRODUCTION. The development of unified techniques for quality control monoclonal antibodies (mAb) is one of the challenges for mAbs quality standardization.AIM.
O. A. Vaganova, R. S. Smirnov, S. A. Lenin, K. S. Funtashev, A. A. Kostromskih, A. S. Peregudova, D. A. Kadykova, V. A. Merkulov   +7 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Multidimensional journal evaluation : analyzing scientific periodicals beyond the impact factor [PDF]

, 2012
Scientific communication depends primarily on publishing in journals. The most important indicator to determine the influence of a journal is the Impact Factor. Since this factor only measures the average number of citations per article in a certain time
Haustein, Stefanie
core   +1 more source

Analysis of a sociological survey on information and educational needs of specialists in the field of medicines circulation as regards the issue of medicines interchangeability

Регуляторные исследования и экспертиза лекарственных средств, 2018
The article summarises the results of a 2015 survey of healthcare professionals and pharmacists that was aimed to reveal their educational needs on the subject of interchangeable medicines.
N. G. Goloenko, R. I. Yagudina, B. K. Romanov, R. N. Alyautdin, D. G. Karapetyan, E. G. Zelenova, E. E. Arinina, A. Yu. Kulikov, M. V. Protsenko, G. T. Abdrashitova   +9 more
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