Results 111 to 120 of about 951,836 (308)
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
Medical Laboratory Journal, 2012 Background and objectives: Tuberculin is the proteins existed in tuberculosis culture medium which precipitated by trichloroacetic acid (TCA) or ammonium sulfate. Tuberculin is used for diagnosis of Tuberculosis.
Sadeghi D (MSc) +7 more
doaj
Colloquium Agrariae, 2012 The female gamete’s microenvironment is the follicular fluid. The proteins in the follicular fluid must interfere in the oocyte quality. The objective of this study was to verify the protein profile in the follicular fluid of different phases of ...
Renato Weller Ribeiro +8 more
doaj +1 more source
SDS-PAGE gel electrophoresis v3 [PDF]
, 2020 SDS-PAGE gel electrophoresis protocol for analyzing samples from plant leaf tissue via immunofluorescence. In this protocol no Coomassie blue is added to samples, the reason is that this interferes with the fluorescent signal during immunoblot. Instead, samples have already been prepared in Laemmli buffer (minus coomassie, protein extraction procedure),
openaire +1 more source
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
, 2017 SDS-PAGEusing gels with low crosslinking of acrylamide and bisacrylamide. This protocol can be used to separate phosphorylation forms of KaiC proteins.
Anika Wiegard +1 more
openaire +1 more source
Isolation and Characterization of Concanavalin A from the seeds of Canavalia ensiformis [PDF]
, 2012 Concanavalin A was isolated from Jackbean (Canavalia ensiformis) seeds. The protein was purified by using affinity chromatography. The activity of the Con A was determined by haemagglutinin assay and the purity of the protein was tested by Sodium dodecyl
Marndi, Rekha
core
FEBS Open Bio, EarlyView.ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand +2 more
wiley +1 more source
Mljekarstvo, 2013 The aim of this work was to investigate primary proteolysis of white brined goat cheese prepared from raw milk and to correlate with the results obtained with high-molarity Tris buffer electrophoretic system.
Milenko Smiljanić +5 more
doaj
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
FEBS Open Bio, EarlyView.Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source