Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Food Technology and Biotechnology, 2005 A total of 65 identified isolates of coliform bacteria Salmonella, including Campylobacter and Staphylococcus isolated from different control points of a poultry slaughterhouse in Ankara, Turkey were characterized by morphological, biochemical and ...
Ayhan Temiz +4 more
doaj
SDS-PAGE Gel Electrophoresis v1 [PDF]
, 2019 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to separate proteins with relative molecular mass no smaller than 10 KD. Very small proteins (<10 KD) are difficult to resolve due to low ability of binding to SDS, which can be solved by gradient gels or using different eletrophoresis conditions, like Tricine-SDS-page.
openaire +1 more source
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. [PDF]
Methods Protoc, 2023 Lim DW +5 more
europepmc +1 more source
The refolding of recombinant human liver methylmalonyl-CoA mutase from inclusion bodies produced in Escherichia coli : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]
, 1998 Human methylmalonyl-CoA mutase (hMCM) is an adenosylcobalamin-dependent enzyme that catalyses the structural rearrangement of (R)-methylmalonyl-CoA to succinyl-CoA as pan of the catabolism of the branched chain amino acids valine, leucine and isoleucine,
Hayes, Michelle Marie
core
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
LAI TẠO VÀ TUYỂN CHỌN DÒNG NẾP THAN THƠM NGẮN NGÀY CÓ NĂNG SUẤT CAO, PHẨM CHẤT TỐT
Tạp chí Khoa học Đại học Cần Thơ, 2010 Từ giống Nếp Than thoái hóa ban đầu thu thập tại tỉnh Sóc Trăng và Trà Vinh, sử dụng kỹ thuật lai tạo theo phương pháp truyền thống, chọn lọc cá thể nhiều lần kết hợp với kỹ thuật điện di protein SDS-PAGE giống đã rút ngắn thời gian sinh trưởng (cải ...
Nguyễn Phúc Hảo +2 more
doaj
SDS-PAGE-Based Quantitative Assay of Hemolymph Proteins in Honeybees: Progress and Prospects for Field Application. [PDF]
Int J Mol Sci, 2023 Isani G +5 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source