SDS-PAGE analysis of rGmolGOBP2 wild-type (WT) and mutants T9A, V111A and V114A.
, 2016 SDS-PAGE analysis of rGmolGOBP2 wild-type (WT) and mutants T9A, V111A and V114A.
Guangwei Li (822910) +5 more
core +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Purification of lipopeptide biosurfactant extracts obtained from a complex residual food stream using Tricine-SDS-PAGE electrophoresis. [PDF]
Front Bioeng Biotechnol, 2023 Moldes AB +3 more
europepmc +1 more source
, 2016 Panel A: Reducing SDS-PAGE, silver-stained. Panel B: Non-reducing SDS-PAGE, silver-stained. Panel C: Non-reducing SDS-PAGE and Western-Blot, immune-staining using the reduction-sensitive monoclonal anti-PfAMA1 antibody 4G2.
Hubert Mertens (3190524) +13 more
core +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
SDS-PAGE Protein and HPTLC Polyphenols Profiling as a Promising Tool for Authentication of Goldenrod Honey. [PDF]
Foods, 2022 Dżugan M +5 more
europepmc +1 more source
SDS-PAGE of the recombinant ATII-TrxR protein.
, 2019 Aliquots from the HisTag purification process of the ATII-TrxR protein from total bacterial proteins were analysed by 10% SDS-PAGE as described in the experimental procedures.
Mahmoud M. Said (6786266) +5 more
core +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
FEBS Open Bio, EarlyView.This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source
Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE. [PDF]
Bio Protoc, 2021 Weinberger Ii J, Lennon CW.
europepmc +1 more source
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source