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Sample preparation by SDS/PAGE and in-gel digestion
2000A convenient method for the isolation of samples for microsequencing or mass spectrometry utilizing polyacrylamide gel electrophoresis is described in detail, including pre-requisites for a successful outcome. Briefly, after visualization by e.g. Coomassie or silver, internal peptides are generated by in-gel proteolytic digestion, and after extraction,
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Protein Determination by SDS-PAGE
SDS-PAGE is the abbreviated form of sodium-dodecyl sulphatepolyacrylamide gel electrophoresis. It is a commonly used gel electrophoresis for the qualitative estimation of proteins. It is based on the principle that treatment of protein with SDS, an anionic detergent, causes a constant negative charge throughout the protein surface.openaire +1 more source
Detergent binding explains anomalous SDS-PAGE migration of membrane proteins
Proceedings of the National Academy of Sciences of the United States of America, 2009Arianna Rath +2 more
exaly
Species identification by SDS-PAGE of red algae used as seafood or a food ingredient
Food Chemistry, 2001Marc Jérôme +2 more
exaly