SDS-PAGE analysis of eluates from mannan-agarose affinity column.
, 2019 Plasma samples (5 mL) from Alligator and Common Snapping Turtles were filtered through a mannan-agarose affinity column in the presence of 20 mM Ca2+. Samples were eluted using EDTA buffer, concentrated and desalted using microcentrifugal concentrators ...
Sarah Baker (813920) +3 more
core +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo +6 more
wiley +1 more source
LAI TẠO VÀ TUYỂN CHỌN DÒNG NẾP THAN THƠM NGẮN NGÀY CÓ NĂNG SUẤT CAO, PHẨM CHẤT TỐT
Tạp chí Khoa học Đại học Cần Thơ, 2010 Từ giống Nếp Than thoái hóa ban đầu thu thập tại tỉnh Sóc Trăng và Trà Vinh, sử dụng kỹ thuật lai tạo theo phương pháp truyền thống, chọn lọc cá thể nhiều lần kết hợp với kỹ thuật điện di protein SDS-PAGE giống đã rút ngắn thời gian sinh trưởng (cải ...
Nguyễn Phúc Hảo +2 more
doaj
SDS-PAGE of purified PmGBE13 (lane 1) and PmGBE57 (lane 2). M: protein standard.
, 2019 SDS-PAGE of purified PmGBE13 (lane 1) and PmGBE57 (lane 2). M: protein standard.
Xuewen Zhang (1774840) +2 more
core +1 more source
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
FEBS Open Bio, EarlyView.Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
SDS-PAGE-Based Quantitative Assay of Hemolymph Proteins in Honeybees: Progress and Prospects for Field Application. [PDF]
Int J Mol Sci, 2023 Isani G +5 more
europepmc +1 more source
SDS-PAGE analysis of rGmolGOBP2 wild-type (WT) and mutants T9A, V111A and V114A.
, 2016 SDS-PAGE analysis of rGmolGOBP2 wild-type (WT) and mutants T9A, V111A and V114A.
Guangwei Li (822910) +5 more
core +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module. [PDF]
Methods Protoc, 2023 Lim DW +5 more
europepmc +1 more source