Results 71 to 80 of about 4,922,198 (308)
SDS-PAGE and Western blot analysis of expressed 8mer-peptides.
, 2022 (A, C, E and G) SDS-PAGE analysis of expressed 8mer-peptides. It indicates each 8mer peptide of P3, P8, P9, P10, P12, P15, P19, P23, P29, P38 and P54. The cell proteins of each recombinant clone were resolved by 12% SDS-PAGE and stained with Coomassie ...
Boyong Jiang (13155018) +8 more
core +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Jurnal Teknologi dan Industri Pangan, 2012 The aim of this research was to extract proteins from the copra meal, base on their solubility and analysis of the protein profiles by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS PAGE) This research was conducted in two stages ...
Agus Danang Wibowo1) +2 more
doaj +2 more sources
Journal of Crop Protection, 2016 This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated
Samira Shahbazi +2 more
doaj
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
, 2022 This protocol shows the steps carried out by team Tec-Chihuahua to perform SDS-PAGE
openaire +1 more source
Separating proteins using SDS Page
, 2011 An articulate presentation detailing the theory and practice of protein separation using SDS PAGE. This unit includes a video demonstration of how to set up and run an SDS PAGE gel.
Dr Sue Whittle, Dr Sue Bickerdike
core
SDS-PAGE analysis of the purified Fabs.
, 2019 Purified Fabs 2A6 and 4E2 were preheated and then individually resolved by SDS-PAGE under denaturing and reducing conditions. The heterodimer was dissociated into the light chain (28 kDa) and the Fd fragment (32 kDa). Lane M represents the protein marker.
Yuting Xian (6883502) +6 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source