Results 111 to 120 of about 5,134,596 (298)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A dynamical model of Kara Sea land‐fast ice [PDF]

Journal of Geophysical Research: Oceans, 2016
AbstractThis paper introduces modifications to the traditional viscous‐plastic sea‐ice dynamical model, which are necessary to model land‐fast ice in the Kara Sea in a realistic manner. The most important modifications are an increase in the maximum viscosity from the standard value of to , and to use a solver for the momentum equation capable of ...
openaire   +2 more sources

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Intra‐population variation in the effects of sea ice reduction on an Arctic breeding bird

Ecosphere
The Arctic is warming four times faster than any other region on Earth, leading to a dramatic reduction in sea ice. Even though sea ice plays a key role in the ecology of many Arctic species, few studies have assessed the consequences of its ...
Hilde Dørum, Sébastien Descamps, Børge Moe, Bård‐Jørgen Bårdsen, Kjell Tore Hansen, Sveinn Are Hanssen, Christophe Sauser, Marika Marnela, Sebastian Gerland, Geir W. Gabrielsen   +9 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Trimlines, blockfields and the vertical extent of the last ice sheet in southern Ireland [PDF]

, 2015
The research reported here was supported by UK NERC grant NER/B/S/2001/0919Trimlines separating glacially abraded lower slopes from blockfield-covered summits on Irish mountains have traditionally been interpreted as representing the upper limit of the ...
Stone, John O., John O. Stone, Ballantyne, Colin, Colin K. Ballantyne   +3 more
core   +1 more source

Drift-aware sea ice thickness maps from satellite remote sensing [PDF]

The Cryosphere
The standard approach to deriving gridded sea ice thickness (SIT) from satellite altimeters is to aggregate the original along-track SIT estimates over a 1-month period to achieve sufficient coverage across the Arctic.
R. Ricker, T. Lavergne, S. Hendricks, S. Paul, E. Down, M. A. Killie, M. Bocquet   +6 more
doaj   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

Oceanographic conditions beneath Fimbul Ice Shelf, Antarctica [PDF]

, 2012
Antarctic ice shelves play a key role in the global climate system, acting as important sites for the cooling of shelf waters, thereby facilitating deep and bottom water formation.
Abrahamsen, Einar Povl
core   +1 more source