Results 131 to 140 of about 4,079,930 (328)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Probability of Sea Ice Extent: Between Eastern Greenland and Iceland 1821-1956

, 2018
Our data set includes nine probability maps of sea ice extent, available in .jpeg and shapfiles. They were created from historical maps of sea ice extent off Iceland dating back to 1877 compiled by Koch (1945) that we digitized.
Karlsson, Nanna Bjørnholt, Hallé, Danielle, Anne M Solgaard (7884506), Solgaard, Anne M, Danielle Hallé (8022431), Camilla S Andresen (7848970), Nanna B Karlsson (7883159), Andresen, Camilla S   +7 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Toward an ice-free Barents Sea [PDF]

, 2017
Arctic winter sea ice loss is most pronounced in the Barents Sea. Here we combine observations since 1850 with climate model simulations to examine the recent record low winter Barents Sea ice extent.
Årthun, Marius, Ingrid H. Onarheim, Onarheim, Ingrid Husøy, Marius Årthun   +3 more
core   +1 more source

Growth and motion at the Weddell Sea ice edge

, 2007
The formation of sea ice in the presence of turbulence was studied using data from drifting buoydeployments and ice sampling in the Weddell Sea during April 2000.
Doble, Martin Jonathan
core   +1 more source

The mechanism linking the variability of the Antarctic sea ice extent in the Indian Ocean sector to Indian summer monsoon rainfall. [PDF]

Clim Dyn, 2023
Azhar SSA, Chenoli SN, Samah AA, Kim SJ, Murukesh N.   +4 more
europepmc   +1 more source

An Initial Assessment of Antarctic Sea Ice Extent in the CMIP5 Models

Journal of Climate, 2013
Abstract This paper examines the annual cycle and trends in Antarctic sea ice extent (SIE) for 18 models used in phase 5 of the Coupled Model Intercomparison Project (CMIP5) that were run with historical forcing for the 1850s to 2005. Many of the models have an annual SIE cycle that differs markedly from that observed over the last 30 ...
Turner, John, Bracegirdle, Thomas J., Phillips, Tony, Marshall, Gareth J., Hosking, J. Scott   +4 more
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A temperate former West Antarctic ice sheet suggested by an extensive zone of bed channels [PDF]

, 2014
We present radio-echo sounding data and satellite imagery, revealing a series of ancient large sub-parallel subglacial channels preserved in the region between the Möller and Foundation Ice Streams, West Antarctica.
Martin J. Siegert, Tom A. Jordan, Rippin, David M., Jordan, Tom A., Ross, N, Siegert, MJ, Corr, HFJ, Siegert, Martin J., Rippin, DM, Le Brocq, Anne, Hugh F.J. Corr, Jordan, TA, Kathryn C. Rose, Le Brocq, Anne M., Rose, Kathryn C., Bingham, RG, Le Brocq, AM, Anne M. Le Brocq, Ross, Neil, Fausto Ferraccioli, Rose, KC, Ferraccioli, F, Ferraccioli, Fausto, Corr, Hugh F.J., Robert G. Bingham, Neil Ross, David M. Rippin, Bingham, Robert G.   +27 more
core   +1 more source