Results 111 to 120 of about 6,117,121 (293)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Expression and localization of Aquaporin 1a in the sea-bass (Dicentrarchus labrax) during ontogeny [PDF]

, 2011
The successful establishment of a species in a given habitat depends on the ability of each of its developing stages to adapt to the environment. In order to understand this process we have studied the adaptation of a euryhaline fish, the sea-bass ...
Guy Charmantier, Charline Abed, Giffard-Mena, Ivone, Guy eCharmantier, Boulo, Viviane, Viviane eBoulo, Ivone Giffard-Mena, Cramb, Gordon, Gordon Cramb, Gordon eCramb, Charmantier, Guy, Charline eAbed, Abed, Charline, Ivone eGiffard-Mena, Viviane Boulo   +14 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Field‐based validation of a diagenetic effect on G. ruber Mg/Ca paleothermometry: Core top results from the Aegean Sea (eastern Mediterranean)

Geochemistry, Geophysics, Geosystems, 2011
Recent work across the Mediterranean Sea has illustrated the salinity and overgrowth effects on planktonic foraminiferal Mg/Ca, which potentially confound the use of this as a temperature proxy for paleoceanographic reconstructions.
George Kontakiotis, P. Graham Mortyn, Assimina Antonarakou, Miguel A. Martínez‐Botí, Maria V. Triantaphyllou   +4 more
doaj   +1 more source

Control of Mode and Intermediate Water Mass Properties in Drake Passage by the Amundsen Sea Low [PDF]

, 2013
The evolution of the physical properties of Subantarctic Mode Water (SAMW) and Antarctic Intermediate Water (AAIW) in the Drake Passage region is examined on time scales down to intraseasonal, within the 1969–2009 period.
Naveira Garabato, Alberto C., Naveira Garabato, Alberto, King, Brian A., McDonagh, Elaine L., Boehme, Lars, Close, Sally, McDonagh, Elaine, King, Brian, Close, Sally E., Biuw, Martin   +9 more
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

SSS Retrieval Using C- and X-Band Microwave Radiometer Observations in Coastal Oceans

Atmosphere
This study proposes a method for retrieving ocean sea surface salinity (SSS) using C/X-band ocean emissivities in coastal regions, aiming to verify the performance of these unconventional frequencies for SSS retrieval in warm, high-salinity-variation ...
Xinyu Li, Xinhao Zuo, Jin Wang
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source