Results 121 to 130 of about 14,708,080 (276)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Neuronal activity in the basolateral amygdala following olfactory threat conditioning, extinction and second order conditioning

This thesis investigates the neuronal activities within the basolateral amygdala (BLA) in response to olfactory threat conditioning, extinction and second order conditioning.
Xia, Yue
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Hyper–Dual Numbers: A Theoretical Foundation for Exact Second Derivatives

Mathematics
Second-order derivative information, including mixed curvature, is central to Newton and trust-region optimization, uncertainty quantification, and simulation-based design.
Sung Bum Park, Ji Eun Kim
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Dentate gyrus and CA3 activity mediates light-tone second-order conditioning expression in mice. [PDF]

iScience
Canela M, Adell JB, Coll DR, González-Parra JA, Pinho JS, Busquets-García A.   +5 more
europepmc   +2 more sources

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Second-order-based blind signal separation in reverberant environments

, 2015
The aim of this paper was to provide a derivation to explain the performance of the second-order-based blind signal separation (BSS) in reverberant environments.
S. Low (23305942), C. Yiu (23407429), Sven Nordholm (23315053)   +2 more
core  

Higher-order pavlovian fear conditioning

, 1973
Higher-order conditioning is a set of operations by which, a neutral stimulus (S) is paired with a conditioned stimulus (CS) in a classical conditioning paradigm.
Fitchett, Susan Mary
core   +1 more source