Results 111 to 120 of about 158,285 (306)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Metabolic engineering of micronutrients in crop plants [PDF]

open access: yes, 2016
Micronutrient deficiency is a widespread phenomenon, most prevalent in developing countries. Being causally linked to the occurrence of a range of diseases, it affects billions of people worldwide. Enhancing the content of micronutrients in crop products
Blancquaert, Dieter   +3 more
core   +2 more sources

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

open access: yesFEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero   +5 more
wiley   +1 more source

Spatio-temporal transcriptome and storage compound profiles of developing faba bean (Vicia faba) seed tissues

open access: yesFrontiers in Plant Science
Faba bean (Vicia faba) is a legume grown in diverse climate zones with a high potential for increased cultivation and use in food due to its nutritional seeds.
Hannah Ohm   +3 more
doaj   +1 more source

Investigation of Seed Storage Proteins in some Wild Wheat Progenitors Using SDS-PAGE and ACID-PAGE

open access: yesNotulae Botanicae Horti Agrobotanici Cluj-Napoca, 2009
Wheat storage proteins accounted for up to 60% of the total grain proteins. They form gluten proteins, which make a visco-elastic network enables dough to be processed into bread, pasta and other products.
Omid SOFALIAN, Mostafa VALIZADEH
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Seed Germination and Initial Stages of Tripogon chinensis (Poaceae) Ontogenesis [PDF]

open access: yesЖурнал Сибирского федерального университета: Серия Биология, 2019
This paper presents the results of research of Tripogon chinensis adaptation mechanisms to the environmental conditions. This relict cereal grows on petrophytic parts of the slopes, where it is affected by extreme environmental factors (temperature ...
Irina N. Plyaskina   +1 more
doaj  

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