Results 111 to 120 of about 5,171,640 (300)

RNA extraction from self-assembling peptide hydrogels to allow qPCR analysis of encapsulated cells.

PLoS ONE, 2018
Self-assembling peptide hydrogels offer a novel 3-dimensional platform for many applications in cell culture and tissue engineering but are not compatible with current methods of RNA isolation; owing to interactions between RNA and the biomaterial.
Kyle A Burgess, Victoria L Workman, Mohamed A Elsawy, Aline F Miller, Delvac Oceandy, Alberto Saiani   +5 more
doaj   +1 more source

Neural stem cells encapsulated in a functionalized self-assembling peptide hydrogel for brain tissue engineering

, 2014
Brain injury is almost irreparable due to the poor regenerative capability of neural tissue. Nowadays, new therapeutic strategies have been focused on stem cell therapy and supplying an appropriate three dimensional (3D) matrix for the repair of injured ...
Cheng, Tzu-Yun;Chen, Ming-Hong;Chang, Wen-Han;Huang, Ming-Yuan;Wang, Tzu-Wei
core   +1 more source

Self-Assembling Peptide Hydrogels for 3D Microarrays

, 2020
Recent advances in biosensing analytical platforms have brought relevant outcomes for novel diagnostic and therapy-oriented applications. In this context, hydrogels have emerged as appealing matrices to locally confine biomolecules onto sensing surfaces ...
Strada A., Gori A., Bergamaschi G., Cretich M., Frigerio R., Strada Alessandro, Bergamaschi Greta, Alessandro Strada, Roberto Frigerio, Frigerio Roberto, Cretich Marina, Alessandro Gori, Greta Bergamaschi, Marina Cretich, Gori Alessandro   +14 more
core   +1 more source

Beyond Primary Nucleation: Molecular Basis of Peptide Crystal Secondary Nucleation at Soft Interface

Advanced Functional Materials, EarlyView.
High spatiotemporal‐resolution atomic force microscopy directly resolved peptide secondary nucleation (SN) and growth characteristics and mechanisms on HOPG. The landscape of peptide nucleation sites, growth dynamics, molecular‐scale structures, phase transitions, and cross‐seeding processes were realized.
Linhao Sun, Abilesh Kumar, Ayhan Yurtsever   +2 more
wiley   +1 more source

A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation

Journal of Nucleic Acids, 2012
Multicomponent RNA-peptide complexes are attractive from the viewpoint of artificial design of functional biomacromolecular systems. We have developed self-folding and self-assembling RNAs that serve as templates to assist chemical ligation between two ...
Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa   +2 more
doaj   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

Furin-Triggered Peptide Self-Assembly Activates Coumarin Excimer Fluorescence for Precision Live-Cell Imaging

Molecules
Monomer-to-excimer transition has become a valuable technique in fluorescence imaging because of its ability to enhance imaging contrast. However, from a practical perspective, the accuracy of excimer formation at target sites warrants further ...
Peiyao Chen, Liling Meng, Yuting Wang, Xiaoya Yan, Meiqin Li, Yun Deng, Yao Sun   +6 more
doaj   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

Enzyme-responsive peptide dendron nanoassemblies for targeting and eliminating intracellular drug-resistant bacteria

Nature Communications
Certain intracellular pathogens can reside within cells to evade host immune defenses and exhibit high tolerance to antibiotics. Current treatments for intracellular bacterial infections are limited by poor cellular penetration, inadequate targeting of ...
Qi Tang, Peng Tan, Chenlong Zhou, Guanghui Zhao, Shuaikang Yang, Yucheng Zhang, Tao Wang, Zhenduo Chen, Xi Ma   +8 more
doaj   +1 more source

Mitochondria localization induced self-assembly of peptide amphiphiles for cellular dysfunction

Nature Communications, 2017
Spatiotemporal control of intracellular molecular self-assembly holds promise for therapeutic applications. Here the authors develop a peptide consisting of a phenylalanine dipeptide with a mitochondrial targeting moiety to form self-assembling fibrous ...
M. T. Jeena, L. Palanikumar, Eun Min Go, Inhye Kim, Myoung Gyun Kang, Seonik Lee, Sooham Park, Huyeon Choi, Chaekyu Kim, Seon-Mi Jin, Sung Chul Bae, Hyun Woo Rhee, Eunji Lee, Sang Kyu Kwak, Ja-Hyoung Ryu   +14 more
doaj   +1 more source