Results 51 to 60 of about 1,347 (276)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Résilience de la lentille au changement climatique au Maroc : principales réalisations en amélioration génétique, biotechnologie et gestion des cultures

African and Mediterranean Agricultural Journal - Al Awamia
Au Maroc, la lentille est cultivée dans des conditions pluviales et sa productivité est fortement influencée par les aléas climatiques, notamment la quantité et la distribution des précipitations, ainsi que les hautes températures.
Abdelmonim Zeroual, Mohammed Mitache, Aziz Baidani, Hanane Ouhemi, Sanae Krimi Bencheqroun, Fatima Zahra Ibn El Mokhtar, Rachid Mentag, Nadia Benbrahim, Omar Idrissi   +8 more
doaj   +1 more source

Avaliação agro-ambiental dos sistemas de cultura sob plantio direto, cultivo mínimo e aração na região de Is sur Tille (Côte d'Or -França) e Ituporanga (SC - Brasil) [PDF]

, 2010
TCC (graduação em Agronomia) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, 2010À partir de alguns anos o GEDA (Grupo de estudos e desenvolvimento agrícolas) da Tille (região do departamento da Côte d'Or - França) e os ...
Beber, Caetano Luiz
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Contribution de la recherche agricole pour une meilleure résilience de l’agriculture marocaine

African and Mediterranean Agricultural Journal - Al Awamia
L’agriculture joue un rôle socio-économique important au Maroc. Elle représente 13 % du Produit National Brut du pays et engage environ 46 % de la population.
Faouzi Bekkaoui, Mohammed Sadiki, Imane Thami Alami   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Lagrangian Mechanics on Centered Semi-direct Products [PDF]

, 2015
Comments Welcome!
Colombo, Leonardo J., Jacobs, Henry O.
openaire   +2 more sources

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Le semis direct au secours de l'exploitation de Solo Koulibaly

, 2005
Solo Koulibaly est un paysan dynamique de la zone cotonnière de Haute Guinée, qui tente de semer ses cultures au plus tôt dans la saison des pluies et obtient habituellement de bons rendements.
Kourouma, Makan, Bozza, Jean-Louis
core  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source