Results 91 to 100 of about 1,009,466 (301)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Racing in Kart Dromes: Laboratory and Site Assessment of Noise Levels from Competition and Rental Karts

Acoustics
Kart racing is one of the hobbies that people get passionate about from a young age. Kart dromes are commonly built in suburban or rural areas, generally surrounded by industrial zones and sporadic residential buildings. The circuits are primarily active
Antonella Bevilacqua, Gino Iannace, Luis Gomez-Agustina, Amelia Trematerra   +3 more
doaj   +1 more source

Access Interfaces for Open Archival Information Systems based on the OAI-PMH and the OpenURL Framework for Context-Sensitive Services

, 2005
In recent years, a variety of digital repository and archival systems have been developed and adopted. All of these systems aim at hosting a variety of compound digital assets and at providing tools for storing, managing and accessing those assets.
Bekaert, Jeroen, Van de Sompel, Herbert
core   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Lessons from overtly-headed exclamatives in Spanish varieties

Isogloss
Inverting wh-exclamative sentences with an overt complementizer in languages like Spanish pose a serious challenge to traditional accounts of obligatory subject-verb inversion.
Julio Villa-García
doaj   +1 more source

Muons tomography applied to geosciences and volcanology

, 2012
Imaging the inner part of large geological targets is an important issue in geosciences with various applications. Dif- ferent approaches already exist (e.g.
Acquafredda, Alvarez, Arrabito, Baringer, Boudon, Bugaev, D. Gibert, F. Giacoppo, F. Nicollin, Gaisser, Gibert, J. Marteau, Komorowski, Lesparre, Marteau, N. Lesparre, Nagamine, Nagamine, Nagamine, Nakamura, Nicollin, P. Noli, Pla-Dalmau, Tanaka, Tanaka, Tanaka, Zichichi   +26 more
core   +3 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A planetary companion around the K giant eps Corona Borealis

, 2012
Aims. Our aim is to search for and study the origin of the low-amplitude and long-periodic radial velocity (RV) variations in K giants. Methods. We present high-resolution RV measurements of K2 giant epsilon CrB from February 2005 to January 2012 using ...
B.-C. Lee, Bertelli, D. E. Mkrtichian, da Silva, de Medeiros, Donati, Döllinger, Döllinger, Fischer, Frink, Gettel, Ghezzi, Girardi, Glazunova, Han, Han, Hatzes, Hatzes, Hatzes, Hekker, I. Han, Ida, Johnson, Johnson, Johnson, Johnson, Jørgensen, K.-M. Kim, Kim, Kürster, Lee, Lomb, Lovis, M.-G. Park, Massarotti, Mitchell, Moutou, Niedzielski, Pasquini, Pasquini, Piskunov, Quirrenbach, Reffert, Reiners, Richichi, Saar, Santos, Sato, Sato, Sato, Scargle, Setiawan, Setiawan, Shaya, Sozzetti, Takeda, Tody, Wittenmyer   +57 more
core   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source