Results 211 to 220 of about 27,096,978 (348)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Global, regional and national population attributable fraction of potentially modifiable risk factors for dementia in the adult population: an analysis using global health databases. [PDF]

BMJ Public Health
Giannoni-Luza S, Urso D, Logroscino G.
europepmc   +1 more source

Vitamin C content and sensorial properties of dehydrated carrots blanched conventionally or by ultrasound.

Food Chemistry, 2013
J. Gamboa‐Santos, A. Cristina Soria, M. Pérez-Mateos, J. A. Carrasco, A. Montilla, M. Villamiel   +5 more
semanticscholar   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Latent transition analysis for longitudinal studies of post-acute infection syndromes. [PDF]

Nat Commun
Gusinow R, Górska A, Canziani LM, Lopes-Rafegas I, Alvarez Garavito C, Tami A, Gentilotti E, Sicuri E, Laouénan C, Ghosn J, Florence AM, Lahfej N, Mazzaferri F, Del Piccolo L, Giannella M, Toschi A, Di Chiara M, Caponcello MG, Palacios-Baena ZR, Wold KI, Rossi E, Tacconelli E, Hasenauer J, ORCHESTRA study group.   +23 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Drills or Distress? Understanding the Psychological Effects of Active Shooter Drills on Adolescents. [PDF]

J Sch Nurs
Temple JR, Baumler E, Owen CP, Wood L.
europepmc   +1 more source

Intercompartmental communication in senescence

FEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz, Eleanor J. Wind, Jixiang Leng, Myriam Gorospe   +3 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Evaluation of Total Volatile Basic Nitrogen, Formaldehyde, and Formic Acid as Markers to Define the Acceptability of Farmed Sea Bass and Sea Bream Stored Under Vacuum (VP) or in Modified-Atmosphere Packaging (MAP) at 4 ± 2 °C. [PDF]

Microorganisms
Pellegrini M, Andyanto D, Iacumin L, Comi G.   +3 more
europepmc   +1 more source