Results 141 to 150 of about 4,640,138 (299)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Development of Coalescence Coupled Hydrocyclone Assembly

Shiyou jixie, 2018
The oil removing effect of hydrocyclone is not only related to the structure size of cyclone pipe, but also to its pretreatment and the type of lifting pump.
Zhao Chongwei, Wang Chungang, Gong Jian, Liu Kaiwen, Cao Yuan   +4 more
doaj  

Effects of different quantities of antibody conjugated with magnetic nanoparticles on cell separation efficiency. [PDF]

Heliyon, 2020
Haghighi AH, Khorasani MT, Faghih Z, Farjadian F.   +3 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Gradient Engineered Light Absorption Layer for Enhanced Carrier Separation Efficiency in Perovskite Solar Cells. [PDF]

Nanoscale Res Lett, 2020
Wu G, Zhu Q, Zhang T, Zou Z, Wang W, Cao Y, Kong L, Zheng X, Wu Y, Li X, Wu Z, Kang J.   +11 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Combining Electrostatic, Hindrance and Diffusive Effects for Predicting Particle Transport and Separation Efficiency in Deterministic Lateral Displacement Microfluidic Devices. [PDF]

Biosensors (Basel), 2020
Biagioni V, Balestrieri G, Adrover A, Cerbelli S.   +3 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Energy Efficiency Board 2017 programs and operations report.

, 2018
Annual; Began with 2010; ceased with 2011.; At head of title: Connecticut Energy Efficiency ...
Connecticut. Energy Efficiency Board.
core  

National Action Plan for Energy Efficiency [PDF]

, 2006
This report presents policy recommendations for creating a national commitment to energy efficiency through gas and electric utilities, utility regulators, and partner ...
National Action Plan for Energy Efficiency (U.S.)
core