Results 31 to 40 of about 124,089 (289)
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
The Iraqi Journal of Agricultural scienceThis study investigated the synergistic effect of glass wool and sephadex sperm filtration techniques on improving the low semen quality of buffalo bulls with or without antioxidants.
Saif N. Alwaeli, S. M. Eidan
doaj +1 more source
Advanced Biology, EarlyView.This study investigates the photosensitizing potential of Passiflora cincinnata in Antimicrobial Photodynamic Therapy (aPDT) against skin infection caused by Methicillin‐resistant Staphylococcus aureus (MRSA) in a senescent murine model. The P. cincinnata exhibits antimicrobial activity, with reduced bacterial load, lower leukocyte infiltration, and ...
Caroline Vieira Gonçalves +14 more
wiley +1 more source
Immobilization of Vibrio cholerae S1 (NAG) L-Glutaminase on Different Supports
مجلة بغداد للعلوم, 2010 Sixteen Vibrio isolates producing L-glutaminase were obtained from clinical and water samples , one isolate was selected according to its’ highest enzyme productivity , it was identified as Vibrio cholerae (NAG) and coded as V. cholerae S1.
Shatha Salman Hassan
doaj +1 more source
Zhongguo youzhi, 2023 为进一步提升南瓜籽多肽的ACE抑制活性，利用Plastein反应对南瓜籽ACE抑制肽进行修饰，探究底物质量分数、反应温度、反应时间以及3种外源氨基酸添加量对ACE抑制率的影响。采用超滤和Sephadex G-25柱层析等分离修饰产物，并利用LC-MS/MS鉴定肽序列。结果表明：Plastein反应修饰的最佳条件为底物质量分数45%，反应温度20 ℃，反应时间3 h；分别添加亮氨酸、苯丙氨酸或甘氨酸均能显著提升修饰产物的ACE抑制率，其中添加0.5 mmol/g亮氨酸时，修饰产物的ACE抑制率最高 ...
胡田媛1,杨晨1，陈哲2,3，郑竟成1,3，何东平1,3，杨永2,3，雷芬芬1,3 HU Tianyuan1, YANG Chen1, CHEN Zhe2,3, ZHENG Jingcheng1,3, HE Dongping1,3, YANG Yong2,3, LEI Fenfen1,3
doaj +1 more source
A neutrophil-dependent pathway for the generation of a neutral peptide mediator: partial characterization of components and control by alpha-1-antitrypsin. [PDF]
, 1974 A biologically active neutral peptide mediator is cleaved from a plasma protein substrate by an alpha-1-antitrypsin-inhibitable serine protease apparently residing on the membrane of the human neutrophil. The peptide mediator has an approximate mol wt of
Austen, KF, Goetzl, EJ, Wintroub, BU
core
Generating Cell Surface Nucleated Hydrogels with an Artificial Membrane‐Binding Transglutaminase
Advanced Functional Materials, EarlyView.Cell‐based therapies require advanced strategies to enhance cell delivery and bioactivity. Cell membrane engineering offers an avenue to impart new functions to delivered cells to boost their viability and function. Here, an artificial membrane‐binding transglutaminase is generated and biophysically characterized.
Rosalia Cuahtecontzi Delint +6 more
wiley +1 more source
Enrichment of Hypermethylated DNA on Chip for Cervical Cancer Detection
Advanced Materials Interfaces, EarlyView.A microfluidic chip coated with surface‐immobilized methyl binding domain 2 (MBD‐2) protein receptors, is able to bind hypermethylated DNA (hmDNA) from a cancer cell line selectively. Quantification by quantitative polymerase chain reaction (qPCR) shows a 30‐fold enrichment in cervical cancer target DNA.
Ruben W. Kolkman +7 more
wiley +1 more source
Journal of Lipid Research, 1978 DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells.
S K Kundu, S K Roy
doaj +1 more source
Isolation and amino acid sequence analysis of a 4,000-dalton dynorphin from porcine pituitary [PDF]
, 1982 A 4,000-dalton dynorphin was isolated from porcine pituitary. It has 32 amino acids (Mr = 3,986), with the previously described heptadecapeptide (now called dynorphin A) at its amino terminus and a related tridecapeptide, dynorphin B, at its carboxyl ...
Fischli, Walter +3 more
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