Results 41 to 50 of about 119,145 (248)
Purification of lipids from nonlipid contaminants on Sephadex bead columns
Journal of Lipid Research, 1966 A simple, rapid column procedure for the removal of nonlipid contaminants from lipid extracts is described. Lipid P is completely recovered and nonlipids completely removed.
Roy E. Wuthier
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Zhongguo youzhi, 2023 为进一步提升南瓜籽多肽的ACE抑制活性，利用Plastein反应对南瓜籽ACE抑制肽进行修饰，探究底物质量分数、反应温度、反应时间以及3种外源氨基酸添加量对ACE抑制率的影响。采用超滤和Sephadex G-25柱层析等分离修饰产物，并利用LC-MS/MS鉴定肽序列。结果表明：Plastein反应修饰的最佳条件为底物质量分数45%，反应温度20 ℃，反应时间3 h；分别添加亮氨酸、苯丙氨酸或甘氨酸均能显著提升修饰产物的ACE抑制率，其中添加0.5 mmol/g亮氨酸时，修饰产物的ACE抑制率最高 ...
胡田媛1,杨晨1，陈哲2,3，郑竟成1,3，何东平1,3，杨永2,3，雷芬芬1,3 HU Tianyuan1, YANG Chen1, CHEN Zhe2,3, ZHENG Jingcheng1,3, HE Dongping1,3, YANG Yong2,3, LEI Fenfen1,3
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Advanced Science, EarlyView.A supramolecular nanocomplex based on phenolic‐modified silk sericin is developed to deliver antioxidant enzymes across the blood–brain barrier and escape lysosomal degradation. This dual‐barrier‐crossing system reduces neuroinflammation and improves cognitive performance in Alzheimer's disease mouse model, offering a promising strategy for protein ...
Doudou Hu +5 more
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Transport of Proteins into Mitochondria [PDF]
, 1980 The mitochondrial ADP/ATP carrier is an integral transmembrane protein of the inner membrane. It is synthesized on cytoplasmic ribosomes. Kinetic data suggested that this protein is transferred into mitochondria in a posttranslational manner.
Allen E. H. +41 more
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Kinetic study of soybean pure powder lecithin hydrolysis using immobilized phospholipase A2
Grasas y Aceites, 2001 Immobilized A2 phospholipase enzyme promotes the hydrolytic reaction of pure powder soybean lecithin releasing a mole of fatty acid from C-2 position. The main purpose of this paper was to determine the kinetic parameters of this reaction when the enzyme
B. Maroto, C. Camusso, N. Zaritzky
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Advanced Intelligent Discovery, EarlyView.Exosomes are emerging as powerful biomarkers for disease diagnosis and monitoring. This review highlights the integration of surface‐enhanced Raman spectroscopy with artificial intelligence to enhance molecular fingerprinting of exosomes. Machine learning and deep learning techniques improve spectral interpretation, enabling accurate classification of ...
Munevver Akdeniz +2 more
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Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: an in vitro study
Asian Pacific Journal of Tropical Biomedicine, 2014 Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1).
Hakan Söyüt +2 more
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Animal models of chronic thromboembolic pulmonary hypertension
Animal Models and Experimental Medicine, EarlyView.Current animal models of CTEPH. Created using BioRender.com. Abstract Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare, yet life‐threatening disorder characterized by persistent pulmonary vascular obstruction and elevated pulmonary artery pressure, with progressive remodeling and subsequent right heart failure.
Yong‐Jian Zhu +5 more
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Isolation and amino acid sequence analysis of a 4,000-dalton dynorphin from porcine pituitary [PDF]
, 1982 A 4,000-dalton dynorphin was isolated from porcine pituitary. It has 32 amino acids (Mr = 3,986), with the previously described heptadecapeptide (now called dynorphin A) at its amino terminus and a related tridecapeptide, dynorphin B, at its carboxyl ...
Fischli, Walter +3 more
core
Trypsin inhibitor from starchy endosperm of rye seeds
Acta Societatis Botanicorum Poloniae, 2015 A method for the preparation of a trypsin inhibitor from starchy endosperm of rye seeds is described. Isolation and purification comprised the following steps: salting out of the water-soluble proteins at pH 4.5 with ammonium sulphate (0.4 saturation ...
A. Pawłowski
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