Results 41 to 50 of about 78,320 (181)

Purification of lipids from nonlipid contaminants on Sephadex bead columns

Journal of Lipid Research, 1966
A simple, rapid column procedure for the removal of nonlipid contaminants from lipid extracts is described. Lipid P is completely recovered and nonlipids completely removed.
Roy E. Wuthier
doaj   +1 more source

EFFECT OF FILTRATION OF POST-DILUTED SEMEN BY SEPHADEX ON FREEZING ABILITY AND FERTILIZING CAPACITY OF BUFFALO SEMEN [PDF]

Journal of Animal and Poultry Production, 2013
This study aimed to evaluate the effect of Sephadex column filtration technique of post-diluted semen on freezing ability and fertilizing capacity of buffalo semen. Four Egyptian buffalo bulls were used for semen collection.
M. El-Sharawy, A. Hussein, E. El-Siefy, M. El-Sherbieny   +3 more
doaj   +1 more source

Spatiotemporally Controlled Sequential Chemo‐Immunotherapy Activates Pyroptosis for Robust Anti‐Tumor Immunotherapy

Advanced Science, EarlyView.
A glutathione and ultrasound dual‐responsive cerasome achieves sequential co‐delivery of gemcitabine and BMS1166 in tumors. Chemotherapy priming followed by on‐demand PD‐L1 blockade synergistically amplifies anti‐tumor immunity, inhibiting primary tumor growth, distant metastasis and inducing durable immune memory.
Suhui Sun, Ruiqi Wu, Qingshuang Tang, Yunli Xu, Wanrui Shi, Mengxin Wang, Haonan Wang, Yan Luo, Cheng Ma, Xiaolong Liang   +9 more
wiley   +1 more source

Drug-Plasma Binding Measured by Sephadex

, 1962
A method is described using the cross-linked dextran gel, Sephadex, for the study of drug binding. Although not quantitative in terms of per cent bound, this method gives an indication of the comparative strength of binding. The usefulness of this method
C F Barlow, H Firemark, L J Roth
core   +1 more source

EFFECT OF SEPHADEX FILTRATION OF RAM SEMEN ON ITS FREEZABILITY AND FERTILITY [PDF]

Journal of Animal and Poultry Production, 2007
Semen of 10 healthy mature crossbred (½ FR) rams was frozen in Tris-egg yolk glycerol extender before and after filtration through sephadex column G25-150 to investigate the effect of filtration on freezability and fertility outcomes during different ...
B. El-Saidy, M. Al-Gindy, I. Abd El-Razek, M. Ibrahim, A. Metwally, I. El-Shamaa,   +5 more
doaj   +1 more source

Huntingtin Aggregate‐Responsive Autophagy Gene Circuit Mitigates Disease Pathology in R6/2 Mice

Advanced Science, EarlyView.
CD98‐mediated receptor‐mediated transcytosis enables LIP‐CD98 nanocarriers to cross the blood–brain barrier and deliver ARAA to neurons. mHTT aggregates activate the 11G–NarX sensor, initiating Auto‐P and Trans‐P signaling through the VP48–NarL relay.
Jie Zhu, Xi‐xiu Xie, Lei Li, Chen Tian, Hao‐tian Wang, Xiao‐jie Wang, Xiao‐lin Yu, Gui‐feng Zhang, Rui‐tian Liu   +8 more
wiley   +1 more source

Engineering an Artificial Metalloenzyme for Efficient Photo‐Enzymatic CO2 Conversion

Advanced Science, EarlyView.
We built a light‐driven artificial metalloenzyme by covalently conjugating formate dehydrogenase (FDH) with a tailored rhodium complex (Rh‐L6) to overcome spatial‐separation‐limited electron transfer. This integration boosts NADH regeneration and CO2 conversion, giving 2.31‐fold higher formate yield than physical mixtures, due to a narrowed bandgap and
Mengxue Kang, Yan Chu, Zhichun Xu, Jiao Feng, Ganlu Li, Kequan Chen, Hui Li   +6 more
wiley   +1 more source

Kinetic study of soybean pure powder lecithin hydrolysis using immobilized phospholipase A2

Grasas y Aceites, 2001
Immobilized A2 phospholipase enzyme promotes the hydrolytic reaction of pure powder soybean lecithin releasing a mole of fatty acid from C-2 position. The main purpose of this paper was to determine the kinetic parameters of this reaction when the enzyme
B. Maroto, C. Camusso, N. Zaritzky
doaj   +1 more source

南瓜籽ACE抑制肽的Plastein反应修饰及分离鉴定Plastein reaction modification of pumpkin seed ACE inhibitory peptide and its isolation and identification

Zhongguo youzhi, 2023
为进一步提升南瓜籽多肽的ACE抑制活性，利用Plastein反应对南瓜籽ACE抑制肽进行修饰，探究底物质量分数、反应温度、反应时间以及3种外源氨基酸添加量对ACE抑制率的影响。采用超滤和Sephadex G-25柱层析等分离修饰产物，并利用LC-MS/MS鉴定肽序列。结果表明：Plastein反应修饰的最佳条件为底物质量分数45%，反应温度20 ℃，反应时间3 h；分别添加亮氨酸、苯丙氨酸或甘氨酸均能显著提升修饰产物的ACE抑制率，其中添加0.5 mmol/g亮氨酸时，修饰产物的ACE抑制率最高 ...
胡田媛1,杨晨1，陈哲2,3，郑竟成1,3，何东平1,3，杨永2,3，雷芬芬1,3 HU Tianyuan1, YANG Chen1, CHEN Zhe2,3, ZHENG Jingcheng1,3, HE Dongping1,3, YANG Yong2,3, LEI Fenfen1,3
doaj   +1 more source

Anion‐Selective Channels Formed by Synthetic 13‐Mer Chimeric α ‐Peptide‐Oligourea Foldamers

Chemistry – A European Journal, EarlyView.
Synthetic 13‐mer α‐peptide‐oligourea chimeric foldamers self‐assemble into supramolecular ion channels, demonstrating high ion transport activity with distinct anion selectivity in lipid membranes. This study supports the design of bioinspired functional foldamer‐based channels for synthetic ion transport applications.
Chiranjit Dutta, Dandan Su, Pannaga Krishnamurthy, Zhaoxing Li, Jianwei Li, Morgane Pasco, Min Luo, Mihail Barboiu, Gilles Guichard, R. Manjunatha Kini, Prakash Kumar   +10 more
wiley   +1 more source