Results 261 to 270 of about 169,356 (299)

Chromatography of vWF on dextran sulphate sepharose

Thrombosis Research, 1987
A relatively simple and reproducible chromatographic separation using Dextran Sulphate (DS) Agarose is described for the purification of vWf:Ag from cryoprecipitate or plasma source material. The elution profiles suggest high affinity of vWf for the matrix permitting resolution from Fibrinogen, IgG and the unbound Albumin.
R H, Saundry, P, Harrison, G F, Savidge
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Purification of Immunoglobulins Using Protein A-Sepharose

2003
Most immunochemical techniques used in molecular biology rely on a specific class of antibodies, immunoglobulin G (IgG). In mammals, IgG antibodies are produced during the secondary humoral response and contribute about 80% of the serum immunoglobulin. Factors affecting the experimental production of antibodies have been discussed in detail (1).
N J, Kruger, J B, Hammond
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Peptide synthesis on Sepharose^TM beads

The Journal of Peptide Research, 1997
NHS‐activated Pharmacia HiTrap SepharoseTM was modified with 1,3‐diaminopropane to give an amino‐functionalized support suitable for solid‐phase peptide synthesis. The amide linker p‐[(R,S)‐α‐[1‐(9H‐fluoren‐9‐yl)‐methoxyformamido]‐2,4‐dimethoxybenzyl]phenoxyacetic acid was incorporated and the acyl carrier protein sequence 65‐74 was synthesized ...
W, Tegge, R, Frank
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Continuous spectrophotometric assay of a Sepharose-bound enzyme and its use to study kinetics of coupling of the enzyme to sepharose

Analytical Biochemistry, 1973
Abstract Continuous spectrophotometric assay of Sepharose-bound aldolase was performed using a commercial spectrophotometer with a built-in magnetic stirrer. Under most conditions likely to be encountered, the turbidity of the stirred Sepharose suspension did not interfere significantly with the optical measurements.
J S, Mort, D K, Chong, W W, Chan
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Trace adsorption of positively charged proteins onto Sepharose FF and Sepharose FF-based anion exchangers

Journal of Chromatography A, 2012
Agarose-based matrices have been widely used in ion exchange chromatography (IEC). We have herein observed that positively charged proteins (lysozyme and cytochrome c) are adsorbed on the agarose-based anion-exchangers (Q and DEAE Sepharose FF gels) in a capacity of 10-40 μg/mL.
Lin-Ling, Yu, Yan, Sun
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Interferon-sepharose: Induction of the antiviral state

Biochemical and Biophysical Research Communications, 1974
Summary Mouse (L cell) interferon has been covalently attached to Sepharose beads. The interferon-Sepharose beads induce resistance in L cells to virus replication. It is concluded that interferon induces resistance to viral replication from the cell surface only without entry into the cell.
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Heparin-Sepharose Affinity Chromatography

2021
A A, Farooqui, L A, Horrocks
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Sepharose-bound trypsin and activated sepharose-bound trypsinogen

Archives of Biochemistry and Biophysics, 1974
Ralph J. Knights, Albert Light
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Immobilization of human intestinal mucosa enzymes on sepharose

Analytical Biochemistry, 1977
Abstract Digestive enzymes from human intestinal mucosa were solubilized by Triton X-100 and papain and covalently bound to eyanogen bromide-activated Sepharose 4-B gel. Triton X-100 solubilized most of the activities, and 39.1 to 63.5% were immobilized on the carrier. The other enzymes, still bound on the microvilli, were subsequently solubilized by
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Affinitäts-Chromatographie von Plasminogen an Lysin-Sepharose und Concanavalin A-Sepharose

Fresenius' Zeitschrift für analytische Chemie, 1982
E. Köttgen, H. Å. Fabricius, S. Schmitt, W. Mössner, W. Gerok   +4 more
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