Results 251 to 260 of about 169,356 (299)

Enzyme immobilization on palmityl–sepharose

Biotechnology and Bioengineering, 1983
AbstractEnzymes adsorbed on palmityl‐substituted Sepharose 4B by hydrophobic interactions have been used in reactor‐type experiments. Results presented on immobilized glutamate dehydrogenase, trypsin, α‐chymotrypsin, and amyloglucosidase indicate possible potential of the method for continuous catalytic operations.
M, Nemat-Gorgani, K, Karimian
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Immobilization of pepsinogen on Sepharose

Biotechnology Letters, 1984
Porcine pepsinogen was attached to CNBr-activated-Sepharose 4B and self-activated to form Sepharose-pepsin. The maximum loading of pepsin on Sepharose was about 43 mg of pepsin per g of freeze-dried product. Sepharose-pepsin was stable on exposure to protein substrates and had a specific activity similar to that of the free enzyme.
K. M. Shamsuzzaman, N. F. Haard
openaire   +1 more source

Protein purification using combined streptavidin (or avidin)-Sepharose and thiopropyl-Sepharose affinity chromatography

Journal of Chromatography A, 1992
The major problem usually encountered in the application of the (strept)avidin-biotin system to the purification of proteins (or other biological molecules) lies in the difficult reversion of the interaction between immobilized (strept)avidin and the adsorbed biotinylated protein.
F, Desarnaud, J, Marie, R, Larguier, C, Lombard, S, Jard, J C, Bonnafous   +5 more
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Non-specific binding to protein A Sepharose and protein G Sepharose in insulin autoantibody assays may be reduced by pre-treatment with glycine or ethanolamine

Journal of Immunological Methods, 2006
Insulin autoantibody (IAA) microassays are widely used for predicting type 1 diabetes. As levels of IAA are often low in type 1 diabetes, non-specific binding (NSB) needs to be minimised if assays are to achieve high analytical sensitivity.
Alistair Williams, Polly J Bingley
exaly   +2 more sources

Polysome Isolation by Sepharose Column Chromatography

Preparative Biochemistry, 1975
Polysomes from the mouse myeloma MOPC-21 were purified by gel filtration of Sepharose 6B, 4B and 2B columns. All three columns eliminated nearly all intracellular material smaller than 40 S subunits. In addition, passage through 4B and 2B columns substantially reduced the amount of subunits and monosomes in the preparations. Purified polysomes retained
W H, Eschenfeldt, R J, Patterson
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Interactions of Alcohol Dehydrogenase to p‐Hydroxyacetophenone–sepharose and p‐Acetamidophenol‐sepharose

Alcoholism: Clinical and Experimental Research, 2005
Abstract: Background: p‐Hydroxyacetophenone (HAP)‐sepharose is known to be an effective ligand for isolation of aldehyde dehydrogenase and chloramphenicol acetyltransferase. In this study, we investigated ligand specificities to alcohol dehydrogenase (ADH) using p‐HAP‐sepharose and p‐acetamidophenol (AAP)‐sepharose.Methods: p‐HAP and p‐AAP were ...
Munetaka, Negoro, Ichiro, Wakabayashi
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Selective arginase purification by Sepharose immunoabsorbent

Analytical Biochemistry, 1975
Abstract This work has shown that the method of “affinity chromatography” with antiarginase-Sepharose succesfully purifies arginase from semipurified liver extract with high yields and reproducibility.
R, Tarrab, B, Pérez, C, López
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Adsorption Performance of Proteins to CM Sepharose FF and DEAE Sepharose FF Adsorbents

Korean Journal of Chemical Engineering, 2003
Adsorption equilibrium and kinetics were studied for the binding of proteins to CM Sepharose FF and DEAE Sepharose FF. The influence of temperature, pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics was investigated in detail.
Shan-Jing Yao, Yi-Xin Guan, Li-Hua Yu
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Induction of tyrosine aminotransferase by Sepharose-insulin

Archives of Biochemistry and Biophysics, 1974
Abstract Insulin covalently bound to Sepharose causes a nearly 2-fold increase in tyrosine aminotransferase activity in monolayer cultures of hepatoma cells previously incubated with dexamethasone. The time course of the induction and its resistance to inhibition by actinomycin D is similar to that obtained with free insulin, although approximately ...
J L, Garwin, T D, Gelehrter
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Preparation of sepharose-bound poly (rI:rC)

Biochemical and Biophysical Research Communications, 1971
Abstract Poly rI was covalently bound through its terminal 5′-phosphate moiety to Sepharose and then annealed with poly rC to yield insoluble matrix bound poly (rI:rC). The method has no serious deleterious effects on the integrity or in vitro biological activity of the double stranded complexes.
A F, Wagner, R L, Bugianesi, T Y, Shen
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