Results 311 to 320 of about 240,931 (342)

Iron transport from sepharose-bound transferrin

Biochemical and Biophysical Research Communications, 1977
Abstract To ascertain whether transferrin need enter the reticulocyte to deliver its iron after the association of transferrin with the cell membrane, { 125 I, 59 Fe-}labeled transferrin was covalently bound to Sepharose beads. Iron uptake from Sepharose-bound transferrin into rabbit reticulocytes was about 9% that from free transferrin while heme ...
J, Glass, M T, Nunez, S H, Robinson
openaire   +2 more sources

Preparation of sepharose-bound poly (rI:rC)

Biochemical and Biophysical Research Communications, 1971
Abstract Poly rI was covalently bound through its terminal 5′-phosphate moiety to Sepharose and then annealed with poly rC to yield insoluble matrix bound poly (rI:rC). The method has no serious deleterious effects on the integrity or in vitro biological activity of the double stranded complexes.
A F, Wagner, R L, Bugianesi, T Y, Shen
openaire   +2 more sources

Peptide synthesis on Sepharose^TM beads

The Journal of Peptide Research, 1997
NHS‐activated Pharmacia HiTrap SepharoseTM was modified with 1,3‐diaminopropane to give an amino‐functionalized support suitable for solid‐phase peptide synthesis. The amide linker p‐[(R,S)‐α‐[1‐(9H‐fluoren‐9‐yl)‐methoxyformamido]‐2,4‐dimethoxybenzyl]phenoxyacetic acid was incorporated and the acyl carrier protein sequence 65‐74 was synthesized ...
W, Tegge, R, Frank
openaire   +2 more sources

Interactions of Alcohol Dehydrogenase to p‐Hydroxyacetophenone–sepharose and p‐Acetamidophenol‐sepharose

Alcoholism: Clinical and Experimental Research, 2005
Abstract: Background: p‐Hydroxyacetophenone (HAP)‐sepharose is known to be an effective ligand for isolation of aldehyde dehydrogenase and chloramphenicol acetyltransferase. In this study, we investigated ligand specificities to alcohol dehydrogenase (ADH) using p‐HAP‐sepharose and p‐acetamidophenol (AAP)‐sepharose.Methods: p‐HAP and p‐AAP were ...
Munetaka, Negoro, Ichiro, Wakabayashi
openaire   +2 more sources

Adsorption Performance of Proteins to CM Sepharose FF and DEAE Sepharose FF Adsorbents

Korean Journal of Chemical Engineering, 2003
Adsorption equilibrium and kinetics were studied for the binding of proteins to CM Sepharose FF and DEAE Sepharose FF. The influence of temperature, pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics was investigated in detail.
Shan-Jing Yao, Yi-Xin Guan, Li-Hua Yu
openaire   +1 more source

The Purification of Soybean 11S Globulin with ConA-Sepharose 4B and Sepharose 6B

Agricultural and Biological Chemistry, 1974
(1974). The Purification of Soybean 11S Globulin with ConA-Sepharose 4B and Sepharose 6B. Agricultural and Biological Chemistry: Vol. 38, No. 5, pp. 1083-1085.
Keisuke Kitamura, Kazuyoshi Okubo, Kazuo Shibasaki   +2 more
openaire   +1 more source

Tamisage moléculaire sur Sepharose 6B

Journal of Chromatography A, 1974
Abstract Molecular filtration on Sepharose 6B. Empiric relationships between the distribution coefficient and the Stokes radius on the one hand and the molecular weight on the other of proteins of different degrees of asymmetry The distribution coefficient, K d , of macromolecular proteins as determined by Sepharose 6B filtration is a linear ...
Sylvain Demassieux, Jean-Paul Lachance
openaire   +1 more source

Trace adsorption of positively charged proteins onto Sepharose FF and Sepharose FF-based anion exchangers

Journal of Chromatography A, 2012
Agarose-based matrices have been widely used in ion exchange chromatography (IEC). We have herein observed that positively charged proteins (lysozyme and cytochrome c) are adsorbed on the agarose-based anion-exchangers (Q and DEAE Sepharose FF gels) in a capacity of 10-40 μg/mL.
Lin-Ling, Yu, Yan, Sun
openaire   +2 more sources

Induction of tyrosine aminotransferase by Sepharose-insulin

Archives of Biochemistry and Biophysics, 1974
Abstract Insulin covalently bound to Sepharose causes a nearly 2-fold increase in tyrosine aminotransferase activity in monolayer cultures of hepatoma cells previously incubated with dexamethasone. The time course of the induction and its resistance to inhibition by actinomycin D is similar to that obtained with free insulin, although approximately ...
J L, Garwin, T D, Gelehrter
openaire   +2 more sources

Immobilization of pepsinogen on Sepharose

Biotechnology Letters, 1984
Porcine pepsinogen was attached to CNBr-activated-Sepharose 4B and self-activated to form Sepharose-pepsin. The maximum loading of pepsin on Sepharose was about 43 mg of pepsin per g of freeze-dried product. Sepharose-pepsin was stable on exposure to protein substrates and had a specific activity similar to that of the free enzyme.
K. M. Shamsuzzaman, N. F. Haard
openaire   +1 more source