Results 321 to 330 of about 240,931 (342)

Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose

2007
Microcystin-based affinity matrices have been utilized to demonstrate the association of signaling proteins with protein phosphatases and for the purification of low-abundance microcystin-sensitive protein phosphatases. Here, we describe the procedure for the synthesis and use of microcystin-Sepharose and microcystin-biotin-Sepharose.
Moorhead, Greg B.G., Haystead, Timothy A.J., MacKintosh, Carol   +2 more
openaire   +3 more sources

Cholesterol immobilization via ether-linked Sepharose gels

The Analyst, 1992
Cholesterol has been immobilized on Sepharose-6B via oxyether linkages to the 3- or 25-position. The 3- or 25-hydroxysterol methanesulfonates were coupled with epoxy-Sepharose-6B at 80 degrees C for 24 h. Approximately 2% of the ligand was incorporated into the gel.
R K, Satsangi, G E, Mott
openaire   +2 more sources

Chromatography of vWF on dextran sulphate sepharose

Thrombosis Research, 1987
A relatively simple and reproducible chromatographic separation using Dextran Sulphate (DS) Agarose is described for the purification of vWf:Ag from cryoprecipitate or plasma source material. The elution profiles suggest high affinity of vWf for the matrix permitting resolution from Fibrinogen, IgG and the unbound Albumin.
R H, Saundry, P, Harrison, G F, Savidge
openaire   +2 more sources

MICROFLUOROMETRIC IMMUNOASSAYS WITH ANTIGENS BOUND TO SEPHAROSE BEADS

Annals of the New York Academy of Sciences, 1975
Sepharose beads, to which protein had been coupled, were used in the development of two types of microfluorometric immunoassays. The first is an inhibition assay: a fixed amount of antiserum was absorbed with different amounts of antigen. This was accomplished in both direct and indirect incubation schemes.
Haaijman, J.J., Bloemmen, F.J., Ham, C.M.   +2 more
openaire   +3 more sources

Purification of Immunoglobulins Using Protein A-Sepharose

2003
Most immunochemical techniques used in molecular biology rely on a specific class of antibodies, immunoglobulin G (IgG). In mammals, IgG antibodies are produced during the secondary humoral response and contribute about 80% of the serum immunoglobulin. Factors affecting the experimental production of antibodies have been discussed in detail (1).
N J, Kruger, J B, Hammond
openaire   +2 more sources

Binding of immune complexes to IgA-Sepharose 4B

Journal of Immunological Methods, 1984
A method for the determination and removal of circulating immune complexes in pathological sera was developed using human secretory or dimeric myeloma IgA covalently bound to Sepharose 4B. IgA-Sepharose 4B was able to selectively bind heat or antigen-aggregated human IgG (circulating immune complexes) but not monomeric IgG.
G, Moţa, M A, Dobre
openaire   +2 more sources

Heparin-Sepharose Affinity Chromatography

2021
A A, Farooqui, L A, Horrocks
openaire   +2 more sources

Interferon-sepharose: Induction of the antiviral state

Biochemical and Biophysical Research Communications, 1974
Summary Mouse (L cell) interferon has been covalently attached to Sepharose beads. The interferon-Sepharose beads induce resistance in L cells to virus replication. It is concluded that interferon induces resistance to viral replication from the cell surface only without entry into the cell.
openaire   +2 more sources

Sepharose-bound trypsin and activated sepharose-bound trypsinogen

Archives of Biochemistry and Biophysics, 1974
Ralph J. Knights, Albert Light
openaire   +1 more source

Immobilization of human intestinal mucosa enzymes on sepharose

Analytical Biochemistry, 1977
Abstract Digestive enzymes from human intestinal mucosa were solubilized by Triton X-100 and papain and covalently bound to eyanogen bromide-activated Sepharose 4-B gel. Triton X-100 solubilized most of the activities, and 39.1 to 63.5% were immobilized on the carrier. The other enzymes, still bound on the microvilli, were subsequently solubilized by
openaire   +2 more sources