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Global genomic landscape of <i>Staphylococcus lugdunensis</i>: population structure, antimicrobial resistance, and virulence determinants. [PDF]

Appl Environ Microbiol
Yang D, Du P, Wang F, Fu S, Ni W, Xi W, Zhang Y, Liu C, Gao Z, He Y, Li R.   +10 more
europepmc   +1 more source

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High‐resolution HLA typing for the DQB1 gene by sequence‐based typing

Tissue Antigens, 1998
Abstract: The ideal high‐resolution typing strategy for polymorphic genes is sequence‐based typing. SBT of genomic DNA has been developed for the HLA class H genes DRB1, DRB3/4/5 and DPB1. For the DQB1 gene the sequence‐based typing method was shown to cause a number of problems.
C E, Voorter, M C, Kik, E M, van den Berg-Loonen   +2 more
openaire   +4 more sources

HLA class I sequence-based typing

Human Immunology, 1993
HLA oligogenotyping has been used successfully to characterize most phenotypically undetectable variants of class II genes. Limitations inherent to the class I system have, however, complicated the application of this and other molecular approaches to HLA class I typing.
P, Santamaria, A L, Lindstrom, M T, Boyce-Jacino, S H, Myster, J J, Barbosa, A J, Faras, S S, Rich   +6 more
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HLA‐B locus sequence‐based typing

Tissue Antigens, 1999
We describe an approach for HLA‐B high‐resolution typing. A single locus‐specific amplification generates a 1‐kb fragment useful for direct sequencing. Four internal primers are necessary for exon 2 and 3 cycle‐sequencing in both directions. Fluorescent dye‐labelled nucleotides are incorporated during cycle‐sequencing and reaction products are analyzed
S, Pozzi, A, Longo, G B, Ferrara
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HLA‐DQB1 sequencing‐based typing updated

Tissue Antigens, 2007
AbstractThe use of direct sequencing as a typing strategy is well acknowledged. Direct sequencing identifies all sequence motifs including new polymorphisms in heterozygous sequences. The earlier protocols for human leukocyte antigen HLA‐DQB1 Sequencing‐Based Typing (SBT) frequently encounter preferential amplification of one of the alleles that can ...
A, van Dijk, R, Melchers, M, Tilanus, E, Rozemuller   +3 more
openaire   +2 more sources

GyrA sequence-based typing of Legionella

Medical Microbiology and Immunology, 2000
Comparative sequence analysis of a 423-bp segment of the gyrA gene including a region homologous to the quinolone resistance-determining region (QRDR) of other species was evaluated as a novel typing method for Legionella strains. The study was performed with 29 reference strains representing 11 different Legionella species, with various serogroups ...
A, Feddersen, H G, Meyer, P, Matthes, S, Bhakdi, M, Husmann   +4 more
openaire   +2 more sources

Sequencing-Based Typing of HLA

2007
Human leukocyte antigen (HLA) typing is largely performed by use of PCR-based techniques in patients that require stem cell transplantation. In this chapter, HLA typing by sequencing-based typing techniques is described.
Berthold, Hoppe, Abdulgabar, Salama
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Sequence-Based Typing for Platelet alloantigens

2015
Human platelet antigen (HPA) typing is largely performed by use of DNA-based techniques in patients that require assessing the risk of HPA alloimmunization. In this chapter, HPA typing by sequencing-based typing (SBT) techniques is described.
Shun-Chung, Pai, Liang-In, Lin
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Sequence‐based typing of HLA class II DQB1

Tissue Antigens, 2000
Abstract:Due to the expanding number of known HLA class II DQB1 alleles, high‐resolution oligotyping is becoming ineffective, therefore a sequence‐based typing (SBT) strategy was developed to provide rapid and definitive typing of HLA‐DQB1. HLA‐DQB1*02, *03, *04, *05, and *06 alleles were individually amplified by polymerase chain reaction (PCR) using ...
D M, Dinauer, R A, Luhm, A J, Uzgiris, D D, Eckels, M J, Hessner   +4 more
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