Results 41 to 50 of about 3,380,352 (277)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Improvements in the sequencing and assembly of plant genomes

GigaByte, 2021
Advances in DNA sequencing have made it easier to sequence and assemble plant genomes. Here, we extend an earlier study, and compare recent methods for long read sequencing and assembly.
Priyanka Sharma , Othman Al-Dossary , Bader Alsubaie , Ibrahim Al-Mssallem , Onkar Nath , Neena Mitter , Gabriel Rodrigues Alves Margarido , Bruce Topp , Valentine Murigneux , Ardashir Kharabian Masouleh , Agnelo Furtado , Robert J. Henry   +11 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Assembly Sequence Flexibility – Assembly Precedence Matrix-Based Evaluation Model

IEEE Access
The dimensioning and design of assembly operating equipment (AOE), such as workpiece carriers, assembly devices, and assembly assistive devices, are central to the early phase of many processes in assembly planning.
Marco Bleckmann, Luca Mastroianni, Meret Borgerding, Peter Nyhuis   +3 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

DEGREES OF FREEDOM ANALYSIS IN PRODUCT ASSEMBLING

Al-Qadisiyah Journal for Engineering Science, 2011
Assembly sequence generation is one of the major difficulties in assembly planning process. In this paper, disassembly sequence generation has been used as approach to generate products assembly sequence.
Mahmoud Abbas Mahmoud, Rasha Jabbar Marzoq   +1 more
doaj  

A draft genome sequence for the Ixodes scapularis cell line, ISE6 [version 1; referees: 2 approved]

F1000Research, 2018
Background: The tick cell line ISE6, derived from Ixodes scapularis, is commonly used for amplification and detection of arboviruses in environmental or clinical samples.
Jason R. Miller, Sergey Koren, Kari A. Dilley, Derek M. Harkins, Timothy B. Stockwell, Reed S. Shabman, Granger G. Sutton   +6 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source