Results 101 to 110 of about 2,213,999 (337)
Localization of T cell clonotypes using the Visium spatial transcriptomics platform
STAR Protocols, 2022 Summary: We present a protocol to localize T cell receptor clones using the Visium spatial transcriptomics platform. This approach permits simultaneous localization of both gene expression and T cell clonotypes in situ within tissue sections.
William H. Hudson, Lisa J. Sudmeier
doaj
A One-Year Introductory Biology Majors’ Lab Sequence Incorporating
Journal of Microbiology & Biology Education, 2019 The introduction of Vision and Change by AAAS and the recommendation that biology departments amend their curricula to focus on key concepts and skills necessary for graduates have led to a re-envisioning of introductory curricula across the nation. Many
Merrilee F. Guenther +2 more
doaj +1 more source
FEBS Letters, EarlyView.Hermansky‐Pudlak syndrome type 1 (HPS‐1) is a rare, autosomal recessive disorder with poorly understood renal involvement. Urinary extracellular vesicle (uEV) proteomics and a novel Hps1 mouse model reveal mitochondrial abnormalities and lipid accumulation in HPS‐1 kidney proximal tubule cells. Serum ApoA1 correlates with kidney function in our patient
Dawn M. Maynard +7 more
wiley +1 more source
FEBS Letters, EarlyView.We describe a novel set of Epac‐based FRET‐FLIM biosensors with improved fully cytosolic distribution, achieved without compromising the state‐of‐the‐art performance of our original designs, for detecting cAMP dynamics in real time in live cells with high precision and reliability.
Giulia Zanetti +2 more
wiley +1 more source
Diphthamide synthesis is linked to the eEF2‐client chaperone machinery
FEBS Letters, EarlyView.The diphthamide modification of eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis. We addressed the potential coordination of de novo eEF2 synthesis with simultaneous or subsequent diphthamide modification. Our work reveals that the co‐chaperones Hgh1 and Cpr7, which are known to support folding of nascent ...
Lars Kaduhr +4 more
wiley +1 more source
SIG-DB: leveraging homomorphic encryption to Securely Interrogate privately held Genomic DataBases
, 2018 Genomic data are becoming increasingly valuable as we develop methods to utilize the information at scale and gain a greater understanding of how genetic information relates to biological function.
Flower, Audrey +8 more
core +2 more sources
Bacterial epidemiology and biology - lessons from genome sequencing [PDF]
Genome Biology, 2011 Next-generation sequencing has ushered in a new era of microbial genomics, enabling the detailed historical and geographical tracing of bacteria. This is helping to shape our understanding of bacterial evolution.
Brendan W. Wren, Julian Parkhill
openaire +4 more sources
FEBS Letters, EarlyView.The mitochondrial outer membrane iron–sulphur ([Fe‐S]) protein mitoNEET is a target of the type‐2 diabetes drug pioglitazone. Its unknown molecular function is linked to respiratory complex activity and mitochondrial function. We discovered that O2 protects the mitoNEET [2Fe‐2S] cluster against NO oxidation and desensitization towards reduction by H2S.
Thao Nghi Hoang +9 more
wiley +1 more source
Integer Linear Programming for Sequence Problems: A general approach to reduce the problem size [PDF]
, 2016 Sequence problems belong to the most challenging interdisciplinary topics of the actuality. They are ubiquitous in science and daily life and occur, for example, in form of DNA sequences encoding all information of an organism, as a text (natural or ...
Zörnig, Peter
core
Learning a Hybrid Architecture for Sequence Regression and Annotation
, 2015 When learning a hidden Markov model (HMM), sequen- tial observations can often be complemented by real-valued summary response variables generated from the path of hid- den states. Such settings arise in numerous domains, includ- ing many applications in
Carin, Lawrence +4 more
core +2 more sources