Results 81 to 90 of about 4,078,643 (261)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Protein coexpression using FMDV 2A : effect of “linker” residues [PDF]

, 2013
This article was made open access through BIS OA funding. The research was supported by the MRC.Many biomedical applications absolutely require, or are substantially enhanced by, coexpression of multiple proteins from a single vector.
Minskaia, Ekaterina, Ryan, Martin D.
core   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Protocol for constructing glycan biosynthetic networks using glycowork

STAR Protocols
Summary: Glycans, present across all domains of life, comprise a wide range of monosaccharides assembled into complex, branching structures. Here, we present an in silico protocol to construct biosynthetic networks from a list of observed glycans using ...
Jon Lundstrøm, Luc Thomès, Daniel Bojar   +2 more
doaj   +1 more source

Sequence Alignment in Molecular Biology

, 1998
Molecular biology is becoming a computationally intense realm of contemporary science and faces some of the current grand scientific challenges. In its context, tools that identify, store, compare and analyze effectively large and growing numbers of bio ...
APOSTOLICO, ALBERTO, R. GIANCARLO
core   +1 more source

Genome sequence of the Chlamydophila abortus variant strain LLG [PDF]

, 2011
Chlamydophila abortus is a common cause of ruminant abortion. Here we report the genome sequence of strain LLG, which differs genotypically and phenotypically from the wild-type strain S26/3.
Clark, Ewan M., Smith, David George Emslie, Longbottom, D., David G. E. Smith, Spalding, L., Livingstone, M., Vretou, E., Clark, E.M., David Longbottom, Longbottom, David, Lainson, Alex F., Lainson, F.A., Victoria I. Siarkou, Wheelhouse, Nick M., Morag Livingstone, Spalding, Lucy, Wheelhouse, Nick, Lainson, F Alex, Michelle Sait, Wheelhouse, N., Lucy Spalding, Siarkou, Victoria I, Sait, M., Ewan M. Clark, F. Alex Lainson, Siarkou, V.I., Evangelia Vretou, Siarkou, Victoria I., Smith, David, Sait, Michelle, Clark, Ewan M, Nick Wheelhouse, Livingstone, Morag, Smith, David G E, Smith, D.G.E., Vretou, Evangelia   +35 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Protocol for genetic load analysis in caribou using a modified genomic evolutionary rate profiling

STAR Protocols
Summary: Here, we present a protocol to analyze genetic load in caribou (Rangifer tarandus) using a modified version of the genomic evolutionary rate profiling (GERP) program.
Rebecca S. Taylor, Micheline Manseau, Peng Liu, Paul J. Wilson   +3 more
doaj   +1 more source

AlphaFold-multimer predicts ATG8 protein binding motifs crucial for autophagy research

PLoS Biology, 2023
In this issue of PLOS Biology, Ibrahim and colleagues demonstrate how AlphaFold-multimer, an artificial intelligence–based structure prediction tool, can be used to identify sequence motifs binding to the ATG8 family of proteins central to autophagy ...
Hallvard Lauritz Olsvik, Terje Johansen
doaj  

Guest Editorial: Computational Biology [PDF]

, 2014
Computational biology has been used to help sequence the human genome, create accurate models of the human brain, and assist in modeling biological systems. With the availability of massive datasets, it has also become possible to study different systems
Gupta, Pushpendra K; Ch. Charan Singh University Meerut, UP India
core