Results 131 to 140 of about 2,333,875 (318)

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex [PDF]

, 2005
A single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least nine closely related species.
J. R. Govan, Maiden, Martin C J, Vandamme, Peter, Speert, David P, A. Baldwin, GOVAN, JR, MAIDEN, MCJ, C. G. Dowson, LiPuma, John J., E. Mahenthiralingam, J. J. LiPuma, Baldwin, Adam, HONEYBOURNE, D, Thickett, Kathleen M, Mahenthiralingam, Eshwar, Dowson, Chris G., Thickett, Kathleen M., LIPUMA, JJ, DOWSON, CG, Honeybourne, David, BALDWIN, A, Lipuma, John J, K. M. Thickett, SPEERT, DP, M. C. J. Maiden, D. Honeybourne, Govan, John R., THICKETT, KM, Maiden, Martin C. J., D. P. Speert, P. Vandamme, Govan, John R, Dowson, Chris G, Speert, David P., MAHENTHIRALINGAM, E   +34 more
core   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Diversity and virulence of the genus Cronobacter revealed by multilocus sequence typing (MLST) and comparative genomic analysis

, 2013
Cronobacter spp. (previously known as Enterobacter sakazakii) is a diverse bacterial genus consisting of opportunistic food-borne pathogens affecting all age groups, with particularly severe clinical complications such as meningitis and necrotising ...
Joseph, SM
core  

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

High Rates of Homologous Recombination in the Mite Endosymbiont and Opportunistic Human Pathogen Orientia tsutsugamushi

, 2010
The study was funded by the Wellcome Trust. PS was supported by grants from the Wellcome Trust (073135) and British Infection Society.Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the ...
Feil, EJ, Sonthayanon Piengchan, Wuthiekanun Vanaporn, Stuart D Blacksell, Sharon J Peacock, Blacksell, SD, Bentley Stephen D., Sharon J. Peacock, Bentley, SD, Day, Nicholas P. J., Feil Edward J., Day Nicholas P. J., Stephen D. Bentley, Sonthayanon, P, Chierakul, Wirongrong, Stephen D Bentley, Blacksell, Stuart D., Chierakul Wirongrong, Piengchan Sonthayanon, Stuart D. Blacksell, Day, NP, Bentley, Stephen D., Nicholas P J Day, Holden, MT, Wirongrong Chierakul, Mathew T G Holden, Peacock, Sharon J., Feil, Edward J., Wuthiekanun, Vanaporn, Peacock, SJ, Edward J Feil, Wuthiekanun, V, Nicholas P. J. Day, Sonthayanon, Piengchan, Blacksell, Stuart, Vanaporn Wuthiekanun, Edward J. Feil, Chierakul, W, Peacock Sharon J., Day, Nicholas, Holden, Mathew T. G., Holden, Matthew, Blacksell Stuart D., Holden Mathew T. G., Mathew T. G. Holden   +44 more
core   +2 more sources

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Type Sequence Preserving Heterogeneous Information Network Embedding

, 2019
Lacking in sequence preserving mechanism, existing heterogeneous information network (HIN) embedding discards the essential type sequence information during embedding.
Chen, Wei, Li, Qiang, Chen, Yuxin, Qiu, Zhen, Wang, Tengjiao   +4 more
core   +1 more source