Results 101 to 110 of about 7,026,554 (307)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

A widespread single amino acid mutation in AcrA reduces tigecycline susceptibility in Klebsiella pneumoniae

Microbiology Spectrum
Whole-genome sequencing of a clinical tigecycline non-susceptible Klebsiella pneumoniae strain reveals a novel T188A mutation in AcrA, raising awareness on its role in tigecycline resistance.
Yingchao Xu, Wenjia Wang, Wenya Su, Mingyu Wang, Hai Xu, Xuhua Zhang, Ling Li   +6 more
doaj   +1 more source

On some Euler–Mascheroni type sequences

Computers & Mathematics with Applications, 2010
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
openaire   +2 more sources

Type-sequences of modules

Journal of Pure and Applied Algebra, 2001
Let \(R\) be a Noetherian complete local domain of dimension \(1\). In the present paper, the authors define the type sequence \([r_1, \dots, r_n]\) of a fractional ideal \(M\) of \(R\), where \(r_1\) is the Cohen-Macaulay type of \(M\), and they investigate its behavior.
ONETO, ANNA, ZATINI, ELSA
openaire   +2 more sources

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Xylella fastidiosa sequence types from tree petiole samples.

, 2015
The MLST analysis determined the presence of five clonal sequence types in the District.() Values in parenthesis correspond to alleles and sequence types in this analysis.* asterisk denotes additional loci examined for sequence typing in this analysis ...
Jordan Lee Harris (713777), Yilmaz Balci (713778)   +1 more
core   +1 more source

Genotypic diversity is associated with clinical outcome and phenotype in cryptococcal meningitis across Southern Africa [PDF]

, 2015
This work was supported by Wellcome Trust (UK) (www.wellcome.ac.uk) awards to TB (WT069991), GM (WT098316), and JNJ (WT081794); Medical Research Council (MRC UK) (www.mrc.ac.uk) awards to MCF (K000373/1), RCM (G0601171), TSH (G1002327); European Research
Fisher, Matthew C, Matthew C Fisher, Matthew C. Fisher (129755), Angela Loyse (292490), Meintjes, Graeme, Fisher, Matthew C., Emma J. Robertson (760738), Sabiiti, Wilber, O'Hanlon, SJ, O'Hanlon, Simon J, Joseph N Jarvis, Tihana Bicanic (718812), Thomas S. Harrison (222993), Fisher, MC, Karen M Fuentes-Cabrejo, Fuentes-Cabrejo, KM, Fuentes-Cabrejo, Karen M, Angela Loyse, Fisher, M, Harrison, Thomas S, Harrison, TS, Robertson, EJ, Simon J. O’Hanlon (760740), Loyse, Angela, Meintjes, G, Simon J O'Hanlon, Robin C May, Jarvis, Joseph N, Emma J Robertson, Wilber Sabiiti (170648), Thomas S Harrison, Mathew A. Beale (663616), Wilber Sabiiti, Robertson, Emma J, Joseph N. Jarvis (435279), Robertson, Emma J., Jarvis, JN, May, Robin C, Mathew A Beale, Karen M. Fuentes-Cabrejo (760739), Graeme Meintjes (221054), Beale, Mathew A, Tihana Bicanic, Sabiiti, W, Bicanic, T, May, RC, Loyse, A, May, Robin C; id_orcid, Bicanic, Tihana, Graeme Meintjes, Beale, MA, Robin C. May (170650)   +51 more
core   +1 more source