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Degradome sequencing provides comprehensive insights into RNA degradation profiles. The method described here adapts the 5'-rapid amplification of cDNA ends (5'-RACE) technique, enabling the sequencing of degradome cDNA fragments using short-read next-generation sequencing (NGS).
Shih-Shun, Lin +2 more
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Shih-Shun, Lin +2 more
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SIAM Journal on Algebraic Discrete Methods, 1981
Hammer, P. L., Ibaraki, T., Simeone, B.
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Hammer, P. L., Ibaraki, T., Simeone, B.
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Twenty years of bacterial genome sequencing
Nature Reviews Microbiology, 2015Nicholas J Loman, Mark J Pallen
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Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms
Nature Reviews Microbiology, 2013Tim van Opijnen, Andrew Camilli
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Sequencing depth and coverage: key considerations in genomic analyses
Nature Reviews Genetics, 2014Ian M Sudbery +2 more
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Application of 'next-generation' sequencing technologies to microbial genetics
Nature Reviews Microbiology, 2009Daniel MacLean +2 more
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