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Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family [PDF]

Methods in Molecular Biology, 2017
Assembling multiple DNA fragments into functional plasmids is an important and often rate-limiting step in engineering new functions in living systems.
Femi J Olorunniji, Christine A Merrick, Susan J Rosser   +2 more
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Serine Integrase-based Recombination Enables Direct Plasmid Assembly In Vivo

Synthetic Biology and Engineering, 2023
Wan-Qiu Liu, Fang Ba, Jian Li
exaly   +2 more sources

Site-specific recombination by φC31 integrase and other large serine recombinases

Biochemical Society Transactions, 2010
Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the λ Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently in vitro in the presence of their cognate (~50 bp) phage ...
Margaret C M, Smith, William R A, Brown, Andrew R, McEwan, Paul A, Rowley   +3 more
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Establishing a Serine Integrase‐Based Genetic Memory System In Vitro

Biotechnology and Bioengineering
ABSTRACTThe increasing demand for advanced biosystems necessitates innovative approaches to store and process genetic information. DNA, as a high‐density storage medium, offers a promising solution for creating genetic memory systems that can provide state‐dependent responses to various stimuli. To date, numerous studies have reported on genetic memory
Luyao Wang, Fang Ba, Yufei Zhang, Wan‐Qiu Liu, Jian Li   +4 more
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A diversity of serine phage integrases mediate site-specific recombination in mammalian cells

Molecular Genetics and Genomics, 2006
This study evaluated the ability of five serine phage integrases, from phages A118, U153, Bxb1, phiFC1, and phiRV1, to mediate recombination in mammalian cells. Two types of recombination were investigated, including the ability of an integrase to mediate recombination between its own phage att sites in the context of a mammalian cell and the ability ...
Annahita, Keravala, Amy C, Groth, Sohail, Jarrahian, Bhaskar, Thyagarajan, Jason J, Hoyt, Patrick J, Kirby, Michele P, Calos   +6 more
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Orthogonal Serine Integrases Enable Scalable Gene Storage Cascades in Bacterial Genome

ACS Synthetic Biology
Genome integration enables host organisms to stably carry heterologous DNA messages, introducing new genotypes and phenotypes for expanded applications. While several genome integration approaches have been reported, a scalable tool for DNA message storage within site-specific genome landing pads is still lacking. Here, we introduce an iterative genome
Yufei Zhang, Fang Ba, Shuhui Huang, Wan-Qiu Liu, Jian Li   +4 more
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Reprogrammed Serine Integrases Enable Precise Integration of Synthetic DNA

Summary Despite recent progress in the ability to manipulate the genomes of eukaryotic cells 1–3 , there is still no effective and practical method to precisely integrate large synthetic DNA constructs into desired chromosomal sites using a programmable integrase.
Friedrich Fauser, Sebastian Arangundy-Franklin, Jessica E Davis, Lifeng Liu, Nicola J Schmidt, Luis Rodriguez, Danny F Xia, Nga Nguyen, Nicholas A Scarlott, Rakshaa Mureli, Irene Tan, Yuanyue Zhou, Lynn N Truong, Sarah J Hinkley, Bhakti N Kadam, Stephen Lam, Bryan Bourgeois, Emily Tait, Mohammad Qasim, Vishvesha Vaidya, Adeline Chen, Andrew Nguyen, Yuri R. Bendaña, David A. Shivak, Patrick Li, Andreas Reik, David E Paschon, Gregory D Davis, Jeffrey C Miller   +28 more
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In vivo and in vitro characterization of site-specific recombination of a novel serine integrase from the temperate phage EFC-1

Biochemical and Biophysical Research Communications, 2016
Inki Kim, Hyo-Ihl Chang, Chang Hoon Ha
exaly  

Serine integrase recombinational engineering (SIRE): A versatile toolbox for genome editing

Biotechnology and Bioengineering, 2019
Maarten L De Mol, Anke Goormans, Pieter Coussement   +2 more
exaly  

[Serine-type integrases as tools for genome engineering].

Seikagaku. The Journal of Japanese Biochemical Society, 2013
Nobutaka, Hirano, Mitsuru, Haruki
openaire   +1 more source