Results 61 to 70 of about 248,730 (279)

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Purification of serine proteases from mango (Mangifera indica cv. Chokanan) peel using expanded bed adsorption: optimisation using response surface methodology [PDF]

, 2014
Proteolytic enzymes or proteases are a class of proteins ubiquitously found in all organisms; they act as catalysts and perform diverse vital functions. In plants, proteases of plant origin play a vital function from the mobilisation of storage proteins ...
Abd Manap, Mohd Yazid, Amid, Mehrnoush
core   +1 more source

Pretreatment with serine protease inhibitors impairs Leishmania amazonensis survival on macrophages

Parasites & Vectors
Background Leishmaniases are neglected tropical diseases with great clinical and epidemiological importance. The current chemotherapy available for the treatment of leishmaniasis presents several problems, such as adverse effects, toxicity, long ...
Patrícia de Almeida Machado, Pollyanna Stephanie Gomes, Elaine Soares Coimbra, Herbert Leonel de Matos Guedes   +3 more
doaj   +1 more source

Potential Therapeutic Targeting of Coronavirus Spike Glycoprotein Priming

Molecules, 2020
Processing of certain viral proteins and bacterial toxins by host serine proteases is a frequent and critical step in virulence. The coronavirus spike glycoprotein contains three (S1, S2, and S2′) cleavage sites that are processed by human host proteases.
Elisa Barile, Carlo Baggio, Luca Gambini, Sergey A. Shiryaev, Alex Y. Strongin, Maurizio Pellecchia   +5 more
doaj   +1 more source

Alpha 1 Antitrypsin is an Inhibitor of the SARS-CoV-2–Priming Protease TMPRSS2

Pathogens and Immunity, 2021
Background: Host proteases have been suggested to be crucial for dissemination of MERS, SARS-CoV, and SARS-CoV-2 coronaviruses, but the relative contribution of membrane versus intracellular proteases remains controversial. Transmembrane serine protease
Nurit P Azouz, Andrea Klingler, Victoria Callahan, Ivan Akhrymuk, Katarina Elez, Lluís Raich, Brandon Henry, Justin Benoit, Stefanie Benoit, Frank Noé, Kylene Kehn-Hall, Marc Rothenberg   +11 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Protein targets of inflammatory serine proteases and cardiovascular disease

Journal of Inflammation, 2010
Serine proteases are a key component of the inflammatory response as they are discharged from activated leukocytes and mast cells or generated through the coagulation cascade.
Sharony Ram, Yu Pey-Jen, Park Joy, Galloway Aubrey C, Mignatti Paolo, Pintucci Giuseppe   +5 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Modeling and structural analysis of PA clan serine proteases

BMC Research Notes, 2012
Background Serine proteases account for over a third of all known proteolytic enzymes; they are involved in a variety of physiological processes and are classified into clans sharing structural homology. The PA clan of endopeptidases is the most abundant
Laskar Aparna, Rodger Euan J, Chatterjee Aniruddha, Mandal Chhabinath   +3 more
doaj   +1 more source