Purification of serine proteases from mango (Mangifera indica cv. Chokanan) peel using expanded bed adsorption: optimisation using response surface methodology [PDF]
, 2014 Proteolytic enzymes or proteases are a class of proteins ubiquitously found in all organisms; they act as catalysts and perform diverse vital functions. In plants, proteases of plant origin play a vital function from the mobilisation of storage proteins ...
Abd Manap, Mohd Yazid, Amid, Mehrnoush
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Pretreatment with serine protease inhibitors impairs Leishmania amazonensis survival on macrophages
Parasites & VectorsBackground Leishmaniases are neglected tropical diseases with great clinical and epidemiological importance. The current chemotherapy available for the treatment of leishmaniasis presents several problems, such as adverse effects, toxicity, long ...
Patrícia de Almeida Machado +3 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Alpha 1 Antitrypsin is an Inhibitor of the SARS-CoV-2–Priming Protease TMPRSS2
Pathogens and Immunity, 2021 Background: Host proteases have been suggested to be crucial for dissemination of MERS, SARS-CoV, and SARS-CoV-2 coronaviruses, but the relative contribution of membrane versus intracellular proteases remains controversial. Transmembrane serine protease
Nurit P Azouz +11 more
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Protein targets of inflammatory serine proteases and cardiovascular disease
Journal of Inflammation, 2010 Serine proteases are a key component of the inflammatory response as they are discharged from activated leukocytes and mast cells or generated through the coagulation cascade.
Sharony Ram +5 more
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Potential Therapeutic Targeting of Coronavirus Spike Glycoprotein Priming
Molecules, 2020 Processing of certain viral proteins and bacterial toxins by host serine proteases is a frequent and critical step in virulence. The coronavirus spike glycoprotein contains three (S1, S2, and S2′) cleavage sites that are processed by human host proteases.
Elisa Barile +5 more
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An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Modeling and structural analysis of PA clan serine proteases
BMC Research Notes, 2012 Background Serine proteases account for over a third of all known proteolytic enzymes; they are involved in a variety of physiological processes and are classified into clans sharing structural homology. The PA clan of endopeptidases is the most abundant
Laskar Aparna +3 more
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Viral SERPINS—A Family of Highly Potent Immune-Modulating Therapeutic Proteins
Biomolecules, 2023 Serine protease inhibitors, SERPINS, are a highly conserved family of proteins that regulate serine proteases in the central coagulation and immune pathways, representing 2–10% of circulating proteins in the blood.
Kyle Varkoly +5 more
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