Results 131 to 140 of about 219,974 (370)

Disruption of the blood–brain barrier contributes to neurobehavioral changes observed in rheumatic heart disease

Animal Models and Experimental Medicine, EarlyView.
Sydenham's chorea (SC) is the neurological manifestation associated with acute rheumatic fever (ARF), triggered by a group A streptococcal (GAS) infection. Anti‐GAS antibodies cross‐reacting with tissue proteins are implicated in the development of neurological changes. In this study, using an animal model, we demonstrate that a compromised blood–brain
Rukshan Ahamed Mohamed Rafeek, Riya Thapa, Samarjeet Saluja, Bipandeep Banga, David McMillan, Kadaba Sri Sriprakash, Nicholas M. Andronicos, Adam Hamlin, Natkunam Ketheesan   +8 more
wiley   +1 more source

Capsid Serotype and Timing of Injection Determines AAV Transduction in the Neonatal Mice Brain

PLoS ONE, 2013
Adeno-associated virus (AAV) mediated gene expression is a powerful tool for gene therapy and preclinical studies. A comprehensive analysis of CNS cell type tropism, expression levels and biodistribution of different capsid serotypes has not yet been ...
Paramita Chakrabarty, Awilda M. Rosario, P. Cruz, Zoe B. Siemienski, C. Ceballos‐Diaz, Keith W. Crosby, K. Jansen, D. Borchelt, Ji-yoen Kim, J. Jankowsky, T. Golde, Yona Levites   +11 more
semanticscholar   +1 more source

Perfusion‐Based Production of rAAV via an Intensified Transient Transfection Process

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Increasing demand for recombinant adeno‐associated virus (rAAV)‐based gene therapies necessitates increased manufacturing production. Transient transfection of mammalian cells remains the most commonly used method to produce clinical‐grade rAAVs due to its ease of implementation.
Tam N. T. Nguyen, Damdae Park, Christopher T. Canova, Jose Sangerman, Prasanna Srinivasan, Rui Wen Ou, Paul W. Barone, Caleb Neufeld, Jacqueline M. Wolfrum, Stacy L. Springs, Anthony J. Sinskey, Richard D. Braatz   +11 more
wiley   +1 more source

Molecular Evolution of the Human Enteroviruses: Correlation of Serotype with VP1 Sequence and Application to Picornavirus Classification

Journal of Virology, 1999
Sixty-six human enterovirus serotypes have been identified by serum neutralization, but the molecular determinants of the serotypes are unknown. Since the picornavirus VP1 protein contains a number of neutralization domains, we hypothesized that the VP1 ...
M. Oberste, K. Maher, D. Kilpatrick, M. Pallansch   +3 more
semanticscholar   +1 more source

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndhairo, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Serotype Distribution of Salmonella Isolates from Turkey Ground Meat and Meat Parts

BioMed Research International, 2013
The aim of the study was to find out the serotype distribution of 169 Salmonella colonies recovered from 112 Salmonella positive ground turkey (115 colonies) and 52 turkey meat parts (54 colonies). Out of 15 Salmonella serotypes: S.
I. Erol, M. Goncuoglu, N. Ayaz, L. Ellerbroek, F. S. Bilir Ormanci, Ozlem Iseri Kangal   +5 more
semanticscholar   +1 more source

Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations

Biotechnology Progress, EarlyView.
Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd, Torbjörn Gräslund, Véronique Chotteau   +2 more
wiley   +1 more source

Differential Adeno-Associated Virus Serotype-Specific Interaction Patterns with Synthetic Heparins and Other Glycans

Journal of Virology, 2013
All currently identified primary receptors of adeno-associated virus (AAV) are glycans. Depending on the AAV serotype, these carbohydrates range from heparan sulfate proteoglycans (HSPG), through glycans with terminal α2-3 or α2-6 sialic acids, to ...
M. Mietzsch, F. Broecker, A. Reinhardt, P. Seeberger, R. Heilbronn   +4 more
semanticscholar   +1 more source

Iterative hybrid model based optimization of rAAV production

Biotechnology Progress, EarlyView.
Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller, Gerald Siegwart, Susanne Heider, Michael Sokolov, Angela Botros, Alexandra Umprecht, Moritz von Stosch, Mariano Nicolas Cruz Bournazou   +7 more
wiley   +1 more source

Rational engineering of adeno‐associated virus capsid enhances human hepatocyte tropism and reduces immunogenicity

Cell Proliferation, Volume 55, Issue 12, December 2022., 2022
Transduction efficiency and immune responses hinder the application of recombinant adeno‐associated viral (rAAV) vectors in gene therapy. Here, Han et al. describe a new combined strategy for AAV capsid engineering and obtain several AAV variants with higher liver transduction efficiency and less immune activation.
Jiabao Han, Liyu Zhu, Jingwen Zhang, Lu Guo, Xuehan Sun, Cheng Huang, Kai Xu, Ying Zhang, Wei Li, Qi Zhou   +9 more
wiley   +1 more source