Results 1 to 10 of about 522 (119)

Superior esterolytic activity in environmental Lactococcus lactis strains is linked to the presence of the SGNH hydrolase family of esterases [PDF]

JDS Communications, 2020
Lactococcus lactis strains are widely used in the dairy industry in fermentation processes for production of cheese and fermented milks. However, the esterolytic activity of L. lactis is not generally considered high.
Desirée Román Naranjo, Michael Callanan, Anne Thierry, Olivia McAuliffe   +3 more
doaj   +6 more sources

P. aeruginosa SGNH hydrolase-like proteins AlgJ and AlgX have similar topology but separate and distinct roles in alginate acetylation. [PDF]

PLoS Pathogens, 2014
The O-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. Pseudomonas aeruginosa secretes the anionic, O-acetylated exopolysaccharide alginate during chronic infection in the lungs of ...
Perrin Baker, Tyler Ricer, Patrick J Moynihan, Elena N Kitova, Marthe T C Walvoort, Dustin J Little, John C Whitney, Karen Dawson, Joel T Weadge, Howard Robinson, Dennis E Ohman, Jeroen D C Codée, John S Klassen, Anthony J Clarke, P Lynne Howell   +14 more
doaj   +8 more sources

The active site of the SGNH hydrolase-like fold proteins: Nucleophile–oxyanion (Nuc-Oxy) and Acid–Base zones [PDF]

Current Research in Structural Biology, 2023
SGNH hydrolase-like fold proteins are serine proteases with the default Asp-His-Ser catalytic triad. Here, we show that these proteins share two unique conserved structural organizations around the active site: (1) the Nuc-Oxy Zone around the catalytic ...
Konstantin Denessiouk, Alexander I. Denesyuk, Sergei E. Permyakov, Eugene A. Permyakov, Mark S. Johnson, Vladimir N. Uversky   +5 more
doaj   +4 more sources

Characterization of a GDS(L)-like hydrolase from Pleurotus sapidus with an unusual SGNH motif [PDF]

AMB Express
The GDS(L)-like lipase from the Basidiomycota Pleurotus sapidus (PSA_Lip) was heterologously expressed using Trichoderma reesei with an activity of 350 U L−1. The isoelectric point of 5.0 was determined by isoelectric focusing.
Miriam A. Fingerhut, Lea Henrich, Christiane Lauber, Niklas Broel, Parviz Ghezellou, Dominik Karrer, Bernhard Spengler, Kim Langfelder, Timo Stressler, Holger Zorn, Martin Gand   +10 more
doaj   +5 more sources

Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases [PDF]

Nature Communications
Carbohydrate esterases modify polysaccharides by removing different ester moieties thereby affecting their physicochemical properties and their accessibility by glycoside hydrolases. We determined the full-length structures of two members (Fl8CE20_II and
Michelle Teune, Plínio S. Vieira, Thorben Döhler, Gottfried J. Palm, Theresa Dutschei, Daniel Bartosik, Leona Berndt, Gabriela F. Persinoti, Sandra Maaß, Dörte Becher, Thomas Schweder, Mário T. Murakami, Michael Lammers, Uwe T. Bornscheuer   +13 more
doaj   +2 more sources

Uncovering enzymatic tools promoting lignocellulose breakdown in the anaerobic bacterium Ruminiclostridium cellulolyticum [PDF]

Communications Biology
Understanding the anaerobic deconstruction of recalcitrant lignocellulose remains challenging. Combining substrate composition and transcriptomic analyses, we shortlisted Ruminiclostridium cellulolyticum enzymes that modify lignocelullose and ...
Nicolas Vita, Marion Holmière, Felipe Mejia-Otalvaro, Fabian Debard, Lison Degeilh, Séverine Gagnot, David Crônier, Anouck Habrant, Florian Pion, Yann Denis, Giuliano Sciara, Craig Faulds, Caroline Monteil, Sébastien Santini, Paul-Henri Ducrot, Stéphanie Perret, Véronique Aguié-Béghin, Gaël Panis, Eric Record, Brigitte Chabbert, Henri-Pierre Fierobe   +20 more
doaj   +2 more sources

Discovery, structural characteristics and evolutionary analyses of functional domains in Acinetobacter baumannii phage tail fiber/spike proteins [PDF]

BMC Microbiology
Background The global rise in multidrug-resistant Acinetobacter baumannii infections poses a significant healthcare challenge. Bacteriophage offer a promising alternative to antibiotics for treating A. baumannii infections.
Shenshen Liu, Tao Lei, Yujing Tan, Xiaoyi Huang, Wenxin Zhao, Huanhuan Zou, Jianhui Su, Ji Zeng, Haiyan Zeng   +8 more
doaj   +2 more sources

Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans

Molecules, 2022
Acetylated glucuronoxylan is one of the most common types of hemicellulose in nature. The structure is formed by a β-(1→4)-linked D-xylopyranosyl (Xylp) backbone that can be substituted with an acetyl group at O-2 and O-3 positions, and α-(1→2)-linked 4 ...
Leena Penttinen, Vera Kouhi, Régis Fauré, Tatiana Skarina, Peter Stogios, Emma Master, Edita Jurak   +6 more
doaj   +1 more source

Non-targeted metabonomics and transcriptomics revealed the mechanism of mulberry branch extracts promoting the growth of Sanghuangporus vaninii mycelium

Frontiers in Microbiology, 2022
Sanghuangprous vaninii is a wood-inhabiting fungus, and its mycelium and fruiting body show excellent medicinal values. Mulberry is one of the major hosts of S. vaninii, however, the mechanism of mulberry affecting the growth of S.
Jinxi Huo, Yuqing Sun, Meiliang Pan, Huanyan Ma, Tianbao Lin, Zhiqiang Lv, Yougui Li, Shi Zhong   +7 more
doaj   +1 more source

Understanding the structure and function of GDSL-type esterase/lipase genes in pigeon pea for their role under moisture stress conditions

Plant Stress, 2023
The SGNH hydrolase superfamily's GDSL-type esterase/lipase proteins (GELPs) play a crucial role in plant growth and stress tolerance. However, limited knowledge exists regarding the molecular role of GELP genes under drought conditions, especially in ...
Suman Pahal, Kumari Arpita, Harsha Srivastava, Sandhya Sharma, Sapna Grewal, Kishor Gaikwad   +5 more
doaj   +1 more source