Results 11 to 20 of about 34,392 (132)

Characterization of a GDS(L)-like hydrolase from Pleurotus sapidus with an unusual SGNH motif [PDF]

AMB Express
The GDS(L)-like lipase from the Basidiomycota Pleurotus sapidus (PSA_Lip) was heterologously expressed using Trichoderma reesei with an activity of 350 U L−1. The isoelectric point of 5.0 was determined by isoelectric focusing.
Miriam A. Fingerhut, Lea Henrich, Christiane Lauber, Niklas Broel, Parviz Ghezellou, Dominik Karrer, Bernhard Spengler, Kim Langfelder, Timo Stressler, Holger Zorn, Martin Gand   +10 more
doaj   +6 more sources

A multicopy Y-chromosomal SGNH hydrolase gene expressed in the testis of the platyfish has been captured and mobilized by a Helitron transposon. [PDF]

BMC Genet, 2014
Background: Teleost fish present a high diversity of sex determination systems, with possible frequent evolutionary turnover of sex chromosomes and sex-determining genes.
Tomaszkiewicz M, Chalopin D, Schartl M, Galiana D, Volff JN.   +4 more
europepmc   +8 more sources

Uncovering enzymatic tools promoting lignocellulose breakdown in the anaerobic bacterium Ruminiclostridium cellulolyticum [PDF]

Communications Biology
Understanding the anaerobic deconstruction of recalcitrant lignocellulose remains challenging. Combining substrate composition and transcriptomic analyses, we shortlisted Ruminiclostridium cellulolyticum enzymes that modify lignocelullose and ...
Nicolas Vita, Marion Holmière, Felipe Mejia-Otalvaro, Fabian Debard, Lison Degeilh, Séverine Gagnot, David Crônier, Anouck Habrant, Florian Pion, Yann Denis, Giuliano Sciara, Craig Faulds, Caroline Monteil, Sébastien Santini, Paul-Henri Ducrot, Stéphanie Perret, Véronique Aguié-Béghin, Gaël Panis, Eric Record, Brigitte Chabbert, Henri-Pierre Fierobe   +20 more
doaj   +3 more sources

Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases [PDF]

Nature Communications
Carbohydrate esterases modify polysaccharides by removing different ester moieties thereby affecting their physicochemical properties and their accessibility by glycoside hydrolases. We determined the full-length structures of two members (Fl8CE20_II and
Michelle Teune, Plínio S. Vieira, Thorben Döhler, Gottfried J. Palm, Theresa Dutschei, Daniel Bartosik, Leona Berndt, Gabriela F. Persinoti, Sandra Maaß, Dörte Becher, Thomas Schweder, Mário T. Murakami, Michael Lammers, Uwe T. Bornscheuer   +13 more
doaj   +2 more sources

A Pseudomonas aeruginosa PAO1 acetylcholinesterase is encoded by the PA4921 gene and belongs to the SGNH hydrolase family

Microbiological Research, 2011
Through the use of molecular and biochemical experiments and bioinformatic tools, this work demonstrates that the PA4921 gene of the Pseudomonas aeruginosa PAO1 genome is a gene responsible for cholinesterase (ChoE) activity.
Otero, Lisandro Horacio, Sanchez, Diego German, Hernández, C. Madgalena, Lisa, Angela Teresita, Serra, Ana Luz, Encarnación, Sergio, Domenech, Carlos Eduardo   +6 more
core   +4 more sources

A Csi‐miR3954b‐CsCE70/CsPLA1 Regulatory Module Enhances Citrus Fruit Resistance to Penicillium digitatum by Promoting Phenolic Acid and Flavonoid Biosynthesis [PDF]

Molecular Plant Pathology, Volume 27, Issue 10, October 2026.
Csi‐miR3954b targets CsCE70 and CsPLA1, promoting the synthesis of phenolic acids and flavonoids, thereby enhancing citrus resistance to postharvest green mould. ABSTRACT Green mould is one of the major postharvest diseases affecting citrus. Although evidence indicates that microRNAs (miRNAs) participate in plant defence responses, their roles and ...
Jixin Tian, Xiaoquan Gao, Jialin Chen, Kuo Meng, Ou Chen, Wenjun Wang, Lanhua Yi, Kaifang Zeng   +7 more
wiley   +2 more sources

Discovery, structural characteristics and evolutionary analyses of functional domains in Acinetobacter baumannii phage tail fiber/spike proteins [PDF]

BMC Microbiology
Background The global rise in multidrug-resistant Acinetobacter baumannii infections poses a significant healthcare challenge. Bacteriophage offer a promising alternative to antibiotics for treating A. baumannii infections.
Shenshen Liu, Tao Lei, Yujing Tan, Xiaoyi Huang, Wenxin Zhao, Huanhuan Zou, Jianhui Su, Ji Zeng, Haiyan Zeng   +8 more
doaj   +2 more sources

Evaluation of the Structure-Function Relationship of SGNH Lipase from Streptomyces rimosus by Site-Directed Mutagenesis and Computational Approach. [PDF]

Int J Mol Sci
Streptomyces rimosus extracellular lipase (SrL) is a multifunctional hydrolase belonging to the SGNH family. Here site-directed mutagenesis (SDM) was used for the first time to investigate the functional significance of the conserved amino acid residues ...
Filić Ž, Bielen A, Šarić E, Ćehić M, Crnolatac I, Tomić S, Vujaklija D, Abramić M.   +7 more
europepmc   +2 more sources

Biochemical Characterization of Multimodular Xylanolytic Carbohydrate Esterases from the Marine Bacterium Flavimarina sp. Hel_I_48 [PDF]

ChemBioChem, Volume 26, Issue 13, July 11, 2025.
Multimodular carbohydrate‐active enzymes often combine different catalytic activities for efficient polysaccharide degradation. In this work, the structural and functional characterization of three multimodular xylan‐targeting esterases, Fl6, Fll1 and Fll4 from Flavimarina sp. Hel_I_48, are presented.
Michelle Teune, Thorben Döhler, Daniel Bartosik, Thomas Schweder, Uwe T. Bornscheuer   +4 more
wiley   +2 more sources

Structural analysis of acyltransferases involved in O-antigen modification [PDF]

, 2021
Acyltransferase-3 (AT3) domain-containing proteins are involved in acylation of a diverse range of carbohydrates across all domains of life. In bacteria they are essential in processes including symbiosis, resistance to antimicrobials, and biosynthesis ...
Tindall, Sarah
core   +2 more sources