Results 1 to 10 of about 68,716 (153)

The active site of the SGNH hydrolase-like fold proteins: Nucleophile–oxyanion (Nuc-Oxy) and Acid–Base zones [PDF]

Current Research in Structural Biology, 2023
SGNH hydrolase-like fold proteins are serine proteases with the default Asp-His-Ser catalytic triad. Here, we show that these proteins share two unique conserved structural organizations around the active site: (1) the Nuc-Oxy Zone around the catalytic ...
Konstantin Denessiouk, Vladimir N Uversky, Sergei E Permyakov   +2 more
exaly   +7 more sources

P. aeruginosa SGNH hydrolase-like proteins AlgJ and AlgX have similar topology but separate and distinct roles in alginate acetylation. [PDF]

PLoS Pathogens, 2014
The O-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. Pseudomonas aeruginosa secretes the anionic, O-acetylated exopolysaccharide alginate during chronic infection in the lungs of ...
Perrin Baker, Tyler Ricer, Patrick J Moynihan, Elena N Kitova, Marthe T C Walvoort, Dustin J Little, John C Whitney, Karen Dawson, Joel T Weadge, Howard Robinson, Dennis E Ohman, Jeroen D C Codée, John S Klassen, Anthony J Clarke, P Lynne Howell   +14 more
doaj   +18 more sources

The SGNH hydrolase family: a template for carbohydrate diversity [PDF]

Glycobiology, 2022
The substitution and de-substitution of carbohydrate materials are important steps in the biosynthesis and/or breakdown of a wide variety of biologically important polymers.
Alexander C Anderson, Stefen Stangherlin, Kyle Pimentel   +2 more
exaly   +5 more sources

Superior esterolytic activity in environmental Lactococcus lactis strains is linked to the presence of the SGNH hydrolase family of esterases [PDF]

JDS Communications, 2020
Lactococcus lactis strains are widely used in the dairy industry in fermentation processes for production of cheese and fermented milks. However, the esterolytic activity of L. lactis is not generally considered high.
Desirée Román Naranjo, Michael Callanan, Anne Thierry, Olivia McAuliffe   +3 more
doaj   +6 more sources

Characterization of a GDS(L)-like hydrolase from Pleurotus sapidus with an unusual SGNH motif [PDF]

AMB Express
The GDS(L)-like lipase from the Basidiomycota Pleurotus sapidus (PSA_Lip) was heterologously expressed using Trichoderma reesei with an activity of 350 U L−1. The isoelectric point of 5.0 was determined by isoelectric focusing.
Miriam A. Fingerhut, Lea Henrich, Christiane Lauber, Niklas Broel, Parviz Ghezellou, Dominik Karrer, Bernhard Spengler, Kim Langfelder, Timo Stressler, Holger Zorn, Martin Gand   +10 more
doaj   +6 more sources

Crystallization and preliminary X-ray analysis of a highly stable novel SGNH hydrolase (Est24) from Sinorhizobium meliloti [PDF]

Acta Crystallographica Section F, Structural Biology Communications, 2014
The SGNH hydrolase family includes enzymes that catalyze the hydrolysis of a broad range of substrates. Here, the crystallization and preliminary X-ray crystallographic studies of a novel SGNH hydrolase (Est24) from Sinorhizobium meliloti were performed.
Changsuk Oh, Ramesh Pandian, Kyeong Kyu Kim   +2 more
exaly   +6 more sources

Insights into a water-mediated catalytic triad architecture in CE20 carbohydrate esterases [PDF]

Nature Communications
Carbohydrate esterases modify polysaccharides by removing different ester moieties thereby affecting their physicochemical properties and their accessibility by glycoside hydrolases. We determined the full-length structures of two members (Fl8CE20_II and
Michelle Teune, Plínio S. Vieira, Thorben Döhler, Gottfried J. Palm, Theresa Dutschei, Daniel Bartosik, Leona Berndt, Gabriela F. Persinoti, Sandra Maaß, Dörte Becher, Thomas Schweder, Mário T. Murakami, Michael Lammers, Uwe T. Bornscheuer   +13 more
doaj   +3 more sources

Uncovering enzymatic tools promoting lignocellulose breakdown in the anaerobic bacterium Ruminiclostridium cellulolyticum [PDF]

Communications Biology
Understanding the anaerobic deconstruction of recalcitrant lignocellulose remains challenging. Combining substrate composition and transcriptomic analyses, we shortlisted Ruminiclostridium cellulolyticum enzymes that modify lignocelullose and ...
Nicolas Vita, Marion Holmière, Felipe Mejia-Otalvaro, Fabian Debard, Lison Degeilh, Séverine Gagnot, David Crônier, Anouck Habrant, Florian Pion, Yann Denis, Giuliano Sciara, Craig Faulds, Caroline Monteil, Sébastien Santini, Paul-Henri Ducrot, Stéphanie Perret, Véronique Aguié-Béghin, Gaël Panis, Eric Record, Brigitte Chabbert, Henri-Pierre Fierobe   +20 more
doaj   +3 more sources

Discovery, structural characteristics and evolutionary analyses of functional domains in Acinetobacter baumannii phage tail fiber/spike proteins [PDF]

BMC Microbiology
Background The global rise in multidrug-resistant Acinetobacter baumannii infections poses a significant healthcare challenge. Bacteriophage offer a promising alternative to antibiotics for treating A. baumannii infections.
Shenshen Liu, Tao Lei, Yujing Tan, Xiaoyi Huang, Wenxin Zhao, Huanhuan Zou, Jianhui Su, Ji Zeng, Haiyan Zeng   +8 more
doaj   +3 more sources

Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans [PDF]

Molecules, 2022
Acetylated glucuronoxylan is one of the most common types of hemicellulose in nature. The structure is formed by a β-(1→4)-linked D-xylopyranosyl (Xylp) backbone that can be substituted with an acetyl group at O-2 and O-3 positions, and α-(1→2)-linked 4 ...
Leena Penttinen, Vera Kouhi, Régis Fauré, Tatiana Skarina, Peter Stogios, Emma Master, Edita Jurak   +6 more
doaj   +10 more sources