Results 121 to 130 of about 18,161 (258)

Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures. [PDF]

J Biol Eng
Hanke R, Sieben M, Finger M, Schnoor K, Jeßberger S, Weyand J, de la Fuente LC, Mann M, Azizan A, Kosfeld U, Büchs J.   +10 more
europepmc   +1 more source

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production. [PDF]

Microb Cell Fact, 2019
Heyman B, Lamm R, Tulke H, Regestein L, Büchs J.   +4 more
europepmc   +1 more source

Inhibiting cGAS‐STING to Preserve Mitochondrial–Nuclear Communication and Stemness in Young Tendon Stem Cells: A Hydrogel Strategy against Age‐Related Tendinopathy

Advanced Science, EarlyView.
A dual‐targeting strategy to rejuvenate aged tendons. A reactive oxygen species (ROS)‐responsive hydrogel co‐delivers a selenium nanozyme (scavenges ROS) and a STING inhibitor to tendon stem cells. This combined action restores mitochondria–nucleus communication, alleviates cellular senescence, and rejuvenates tendon regeneration, offering a novel ...
Zhuo Zhang, Weiyong Song, Heng Yin, Yi Wang, Yi Zou, Yuling Li, Yue Luo, Chao Xiang, Xiaoqin Liu, Yan Xiong, Yong Wang, Ke Jiang   +11 more
wiley   +1 more source

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

Advanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han, Boyuan Xue, Guangqi Shan, Meng Meng, Shaojie Wang, Haijia Su   +5 more
wiley   +1 more source

Adjusting Aspergillus niger pellet diameter, population heterogeneity, and core architecture during shake flask cultivation. [PDF]

Biotechnol Biofuels Bioprod
Engelbert K, Deffur C, Cairns TC, Zhang F, Kheirkhah T, Winter H, Junne S, Neubauer P, Briesen H, Meyer V.   +9 more
europepmc   +1 more source

Multi‐Targeting Non‐Specific Genome Engineering in Bacteria

Advanced Science, EarlyView.
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun, Ruixiang You, Yiwen Zhou, Huiyan He, Wenfang Wang, Xudong Qu, Yinhua Lu, Lei Li   +7 more
wiley   +1 more source

A novel multiparameter sensor for shake flask cultivations: Online biomass, dissolved oxygen, and fluorescence monitoring for comprehensive bioprocess characterization. [PDF]

Biotechnol Prog
Strehl L, Kuhn AL, Hoffmann K, Mann M, Magnus JB.   +4 more
europepmc   +1 more source

Production of Cellulase By Shake Flask Method

Turkish Journal of Biology, 1997
Nilüfer GÖNÜL, Ş. Suha SUKAN
openaire   +1 more source

Loss of VMP1 Impairs Tight Junction Recycling and Aggravates Intestinal Barrier Dysfunction in Inflammatory Bowel Disease

Advanced Science, EarlyView.
This study identifies vacuole membrane protein 1 (VMP1) as a critical regulator of intestinal epithelial barrier homeostasis. VMP1 facilitates the recruitment of CORO1C to late endosomes, supporting Retromer‐mediated recycling of the tight junction protein Occludin.
Jiawei Zhao, Jianjun Zou, Chen Zhou, Yingui Wang, Yiman Liu, Yanqing Zhou, Yuxiang Wang, Xinyu Zhang, Huishu Yang, Hongjie Yin, Dongsheng Bai, Yue Zhao, Na Lu   +12 more
wiley   +1 more source

N-1 semi-continuous transient perfusion in shake flask for ultra-high density seeding of CHO cell cultures in benchtop bioreactors. [PDF]

Biotechnol Prog
Lemire L, Reyes SJ, Durocher Y, Voyer R, Henry O, Pham PL.   +5 more
europepmc   +1 more source