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Polysorbate 80 Differentially Impacts Erinacine Production Profiles in Submerged Cultures of <i>Hericium</i>. [PDF]
Smith AV, Zhu H, Mats L, Bozzo G.
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Coffee filters as shake-flask closures
Biotechnology Techniques, 1987Coffee filters are suitable for use as inexpensive and convenient shake-flask closures for propaqating bacteria under aerobic conditions. Gaseous exchange rates are excellent. Sterility is maintained when two layers of filter are used.
Paul A. Hartman, Michael B. Roof
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Static shake-flask biotransformation of endothall
Water Research, 1986Laboratory biotransformation and biodegradation rate coefficients were determined for the aquatic herbicide, endothall. Concentrations of 0.5, 2.0 and 4.0 mg l−1 endothall were employed in a static shake-flask test containing reservoir water. A pseudo-first-order biotransformation rate coefficient of 0.083 day−1 and an aqueous half-life of 8.45 days ...
K.H. Reinert +3 more
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Effective shear rates in shake flasks
Chemical Engineering Science, 2014Abstract In bioprocess development and scale-up and scale-down consistent fermentation conditions in shake flasks and stirred tank reactors are crucial. Fermentation broths at elevated viscosities generally show a non-Newtonian flow behavior. The effective shear rate is known to influence apparent viscosity, and, as a consequence, mixing as well as ...
Heiner Giese +8 more
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Noninvasive measurement of dissolved oxygen in shake flasks
Biotechnology and Bioengineering, 2002AbstractShake flasks are ubiquitous in cell culture and fermentation. However, conventional devices for measuring oxygen concentrations are impractical in these systems. Thus, there is no definitive information on the oxygen supply of growing cells. Here we report the noninvasive, nonintrusive monitoring of dissolved oxygen (DO) in shake flasks using a
Leah, Tolosa +3 more
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Reinventing shake flask fermentation: The breathable flask
Biotechnology and BioengineeringAbstractCultivating cells in shake flasks is a routine operation that is largely unchanged since its inception. A glass or plastic Erlenmeyer vessel with the primary gas exchange taking place across various porous plugs is used with media volumes typically ranging from 100 mL to 2 L.
Vikash Kumar +3 more
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