Results 291 to 300 of about 47,367 (336)

Volumetric power consumption in baffled shake flasks

Chemical Engineering Science, 2006
Abstract For the cultivation of microorganisms, baffled shake flasks are employed when increased levels of oxygenation and mixing are required. Their use has been discouraged, however, due to the danger of a wetted sterile plug and the lower reproducibility of the experimental results.
Cyril P. Peter, Yusuke Suzuki, Kirill Rachinskiy, Stefan Lotter, Jochen Büchs   +4 more
openaire   +1 more source

Validating shaking flasks as representative screening systems

Biochemical Engineering Journal, 2004
The high information demand in biotechnical process development makes shaken bioreactors an essential tool for process development. By far most of all biotechnological experiments are carried out in shaken bioreactors. Nevertheless, the reaction conditions in these systems often are not representative of the following conditions of the technical scale.
Stephan A. Freyer, Matthia König, Andreas Künkel   +2 more
openaire   +1 more source

Parallel substrate feeding and pH-control in shaking-flasks

Biochemical Engineering Journal, 2001
An intermittent feeding system for shaking-flasks was developed to close the gap between batch operated shaking-flasks and fed-batch operated as well as pH-controlled stirred tank reactors. A precise syringe pump was connected via a substrate distribution system to individual 2/2-way miniature valves, one for each of up to 16 shaking-flask. The shaking-
Weuster-Botz, Dirk, Altenbach-Rehm, Jutta, Arnold, Matthias   +2 more
openaire   +3 more sources

High-aeration capacity shake-flask system

Journal of Fermentation and Bioengineering, 1989
Abstract A novel shake-flask designed for high-aeration capacity, the Tunair flask system, was found to be superior to the currently available shake-flasks in the cultivation of selected microorganisms. The significant features of the flasks include wide-mouth opening, barrel-shaped trunk, two battle types, and dome-shaped closure with vanes.
openaire   +1 more source

Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity

Biotechnology and Bioengineering, 2000
In this first article of a series a new method is introduced that enables the accurate determination of the power consumption in a shaking flask. The method is based on torque measurements in the drive and appropriate compensation of the friction losses.
J, Büchs, U, Maier, C, Milbradt, B, Zoels   +3 more
openaire   +2 more sources

Model for oxygen transfer in a shake flask

Biotechnology and Bioengineering, 1978
AbstractThe shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the oxygen supply is becoming a growth‐limiting ...
J. C. Van Suijdam, N. W. F. Kossen, A. C. Joha   +2 more
openaire   +1 more source

Sphalerite bioleaching comparison in shake flasks and percolators

Minerals Engineering, 2019
Abstract Bioleaching experiments with a mixed Acidithiobacillus-culture were carried out in shake flasks as well as in column percolators with crushed and sieved sphalerite-rich ore from a former mine in the German Harz Mountains. Efficient sphalerite dissolution in the bioleaching assays was observed in contrast to the chemical leaching experiments.
Axel Schippers, Christoph Tanne, Jens Stummeyer, Torsten Graupner   +3 more
openaire   +1 more source

In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks

Biotechnology Progress, 2012
AbstractpH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small‐scale culture formats like tissue‐culture flasks and shake flasks. A hydrogel‐based system is reported for in situ pH maintenance without pH measurement in such formats, and is ...
Ketaki, Pradhan, Tejal, Pant, Mugdha, Gadgil   +2 more
openaire   +2 more sources

Fed‐batch mode in shake flasks by slow‐release technique

Biotechnology and Bioengineering, 2006
AbstractMost industrial production processes are performed in fed‐batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode which results in completely different physiological conditions than relevant for production conditions. This may lead to wrong selections of strains.
M, Jeude, B, Dittrich, H, Niederschulte, T, Anderlei, C, Knocke, D, Klee, J, Büchs   +6 more
openaire   +2 more sources

Evaluation and development of static shake-flask biodegradation test systems

Environmental Toxicology and Chemistry, 1983
Abstract Several biodegradation flask systems were evaluated to assess the effects of flask shape and volume and length of incubation on the recovery of 14CO2. During a 6-h incubation period, it was demonstrated that the recovery of 14 CO2 decreased as the volume of the flask (50 ml to 2 L) and the length of the diffusion path increased.
James E. Blum, Cathy A. Stevenson, Dennis M. Stainken   +2 more
openaire   +1 more source