Results 301 to 310 of about 47,367 (336)
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High-yield shake-flask fermentation of xylose to ethanol
Applied Biochemistry and Biotechnology, 1994Methods of achieving high-yield fermentation ofd-xylose to ethanol in shake flasks were investigated using the microaerophilic yeastPichia stipitis NRRL Y-7124. Ethanol yields based on consumed xylose approached the theoretical maximum of 0.51 g/g at high culture volumes.
Brian L. Boynton, James D. McMillan
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Shake Flask to Fermentor: What Have We Learned?
Biotechnology Progress, 1998Initially the concern on scale-up of fermentation processes was with oxygen transfer. Strain stability on scale-up was thought to be sufficiently established by serial transfer in shake flasks where conditions were homogeneous. Unfortunately, we now know that there are often mixing problems in large-scale fermentors.
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Measurement and characterization of mixing time in shake flasks
Chemical Engineering Science, 2011Abstract As yet, investigations on mixing time have been focused on small and large scale stirred tanks. The aim of this study was to develop a new method for characterizing the mixing process and quantitatively measuring the mixing time in shake flasks by introducing a rotating camera for online observation of the fluid flow in combination with a ...
Rung-Kai Tan +2 more
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Controlled release of nutrients to mammalian cells cultured in shake flasks
Biotechnology Progress, 2011AbstractThough cell culture‐based protein production processes are rarely carried out under batch mode of operation, cell line and initial process development operations are usually carried out in batch mode due to simplicity of operation in widely used scale down platforms like shake flasks.
Swati, Hegde +4 more
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High Throughput in Biotechnology: From Shake-Flasks to Fully Instrumented Microfermentors
Recent Patents on Biotechnology, 2009This review provides a broad overview of recent patents and developments in the field of small scale bioreactors. The scope of the work will focus on vessels from about 500 ml down to the submilliliter scale. This field of research is of acknowledged relevance, since the rational use of these devices is contributing to speeding up several areas of ...
Marco P C, Marques +2 more
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Closure effects on oxygen transfer and aerobic growth in shake flasks
Biotechnology and Bioengineering, 2006AbstractOxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of kGa in shake flasks with closures are introduced. Using Pseudomonas putida, microbial growth on glucose
Hossein, Nikakhtari, Gordon A, Hill
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Coimmobilized aerobic/anaerobic mixed cultures in shaked flasks
Journal of Biotechnology, 1996The freely suspended aerobic fungus, Aspergillus awamori, and the anaerobic bacterium, Zymomonas mobilis were cultivated in synthetic medium in a stirred tank reactor. Their immobilized monocultures and coimmobilized mixed cultures were cultivated in shaked flasks in synthetic medium on various substrates.
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Improved shake-flask test for the screening of xanthan-producing microorganisms
World Journal of Microbiology & Biotechnology, 1993Baffled 500 ml Erlenmeyer flasks were compared with conventional 2800 ml Fernbach flasks forXanthomonas campestris to produce xanthan. Bacterial growth rates were similar in both types of flask although the Fernbach flasks gave higher biomass concentrations. Xanthan production was similar in both types of flasks but different viscosities were attained.
E, Galindo +3 more
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Production of High-Titre Diphtheria Toxin in Baffled Shake Flasks
Nature, 1958SUCCESSFUL production of high-titre diphtheria toxin has already been described1, involving aluminium tanks containing 10 or 50 l. of medium. The inoculated medium was stirred at 1,400 r.p.m. so that a vortex was produced, and under these conditions high-titre toxin was obtained in 48 hr.
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