Results 41 to 50 of about 22,410 (289)

Application of an Online-Biomass Sensor in an Optical Multisensory Platform Prototype for Growth Monitoring of Biotechnical Relevant Microorganism and Cell Lines in Single-Use Shake Flasks

Sensors, 2014
In the context of this work we evaluated a multisensory, noninvasive prototype platform for shake flask cultivations by monitoring three basic parameters (pH, pO2 and biomass).
Christian Ude, Jörg Schmidt-Hager, Michael Findeis, Gernot Thomas John, Thomas Scheper, Sascha Beutel   +5 more
doaj   +1 more source

Video1_Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.MP4

, 2023
In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Laura Polomsky (16927947), Marcel Schrader (8927945), Dimitri Ivanov (12238744), Ingo Kampen (16927950), Carsten Schilde (350710), Rainer Krull (3690346), Arno Kwade (1633468), Kathrin Schrinner (8927939)   +7 more
core   +1 more source

Valorization of a Pyrolytic Aqueous Condensate and Its Main Components for L-Malic Acid Production with Aspergillus oryzae DSM 1863

Fermentation, 2022
Pyrolytic aqueous condensate (PAC) might serve as a cost-effective substrate for microbial malic acid production, as it is an unused side stream of the fast pyrolysis of lignocellulosic biomass that contains acetol and acetate as potential carbon sources.
Christin Kubisch, Katrin Ochsenreither
doaj   +1 more source

Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks

Biotechnology Journal
ABSTRACTShake flasks are predominantly used in screening and the early stages of biotechnological process development. However, oxygen‐demanding processes cannot easily be performed in shake flasks, since the maximum oxygen transfer capacity is usually smaller than in stirred reactors.
Schulte, Andreas, Jordan, Andreas, Klöckner, Wolf, Schumacher, Mathias, Corves, Burkhard, Büchs, Jochen   +5 more
openaire   +3 more sources

Liquid state bioconversion of sewage treatment plant sludge in batch fermenter and shake flask

, 2004
A study on liquid state bioconversion of sewage treatment plant (STP) sludge was assisted to evaluate the performance of batch fermenter compared to shake flask in a laboratory.
Alam, Md. Zahangir, Fakhru’l-Razi, Ahmadun   +1 more
core   +1 more source

Video2_Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.MP4

, 2023
In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Laura Polomsky (16927947), Marcel Schrader (8927945), Dimitri Ivanov (12238744), Ingo Kampen (16927950), Carsten Schilde (350710), Rainer Krull (3690346), Arno Kwade (1633468), Kathrin Schrinner (8927939)   +7 more
core   +1 more source

Gram-scale production of recombinant microbial enzymes in shake flasks [PDF]

FEMS Microbiology Letters, 2017
Heterologous production of recombinant proteins is a cornerstone of microbiological and biochemical research as well as various biotechnological processes. Yields and quality of produced proteins have a tremendous impact on structural and enzymology studies, development of new biopharmaceuticals and establishing new biocatalytic processes.
Lukas, Chrast, Radka, Chaloupkova, Jiri, Damborsky   +2 more
openaire   +2 more sources

Video11_Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.MP4

, 2023
In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Laura Polomsky (16927947), Marcel Schrader (8927945), Dimitri Ivanov (12238744), Ingo Kampen (16927950), Carsten Schilde (350710), Rainer Krull (3690346), Arno Kwade (1633468), Kathrin Schrinner (8927939)   +7 more
core   +1 more source

Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks

Frontiers in Bioengineering and Biotechnology, 2021
Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive, time-resolved monitoring of the culture status in shake flasks are lacking.
Nina Ihling, Lara Pauline Munkler, Christoph Berg, Britta Reichenbächer, Johannes Wirth, Dietmar Lang, Roland Wagner, Jochen Büchs   +7 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source