Results 51 to 60 of about 22,410 (289)

Proof-of-concept for a miniaturized shake-flask biopharmaceutical solubility determination by sonic mixing

, 2022
The solvent volume must be very small to obtain biopharmaceutically relevant drug solubility data with low consumption of the solid sample which is often in low supply during early drug development.
Veseli, Ardita, Kristl, Albin, Žakelj, Simon   +2 more
core   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source

Scale up of biopolymer from shake flask to 20l stirred tank fermenter [PDF]

, 2008
This study is about the scaling up for production of poly-βhydroxybutyrate (PHB) from shake flask to 20 liter stirred tank fermenter. This scale-up operation used the method of constant volumetric transfer coefficient (K L a) of oxygen. K L
Dilah, Kadir
core  

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Video7_Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.MP4

, 2023
In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Laura Polomsky (16927947), Marcel Schrader (8927945), Dimitri Ivanov (12238744), Ingo Kampen (16927950), Carsten Schilde (350710), Rainer Krull (3690346), Arno Kwade (1633468), Kathrin Schrinner (8927939)   +7 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A Bioremediation Approach to Mercury Removal in a Shake Flask Culture Using Pseudomonas putida (ATCC 49128)

, 2016
Mercury is one of the most poisonous elements found on earth bonded to sulfhydryl groups of enzymes and proteins, thereby inactivating vital cell functions.
R. M., Yunus, Abdul Aziz, Mohd Azoddein, Mani Malam, Ahmad, Nik Meriam, Nik Sulaiman   +3 more
core   +1 more source

Video8_Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions.MP4

, 2023
In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress ...
Laura Polomsky (16927947), Marcel Schrader (8927945), Dimitri Ivanov (12238744), Ingo Kampen (16927950), Carsten Schilde (350710), Rainer Krull (3690346), Arno Kwade (1633468), Kathrin Schrinner (8927939)   +7 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

PULLULAN PRODUCTION AS AFFECTED BY Aureobasidium pullulans STRAINS AND CULTURING CONDITION.S [PDF]

Journal of Agricultural Chemistry and Biotechnology, 2005
Six pullulan producing strains of Aureobasidium pullulans were obtained and tested (or pull ulan production on three different media. All aureobasidiurn tested strains gave the maximum pullulan production on Reeselev & Jensen medium.
M.S. Sharaf, Hemmat Abdelhady, A.M. Abdel Hafez, T.S. El-Tayeb   +3 more
doaj   +1 more source