Results 51 to 60 of about 17,254 (263)
Shaking during Ion-Atom Collisions [PDF]
, 2015 Shaking (shakeup + shakeoff) probabilities accompanying ion-atom collisions are studied using hydrogenic wavefunctions for K-, L-, M- shell electrons in the sudden approximation limit. The role of recoil velocity in the shaking processes is discussed.
arxiv +1 more source
Molecular Oncology, EarlyView.Triacsin C inhibition of the acyl‐CoA synthetase long chain (ACSL) family decreases multiple myeloma cell survival, proliferation, mitochondrial respiration, and membrane potential. Made with Biorender.com. Multiple myeloma (MM) is an incurable cancer of plasma cells with a 5‐year survival rate of 59%.
Connor S. Murphy +12 more
wiley +1 more source
Biotechnology & Biotechnological Equipment, 2021 2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to ...
Yingxue Liao +6 more
doaj +1 more source
FEBS Open Bio, EarlyView.We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques +5 more
wiley +1 more source
Improved Lycopene Production from Different Substrates by Mated Fermentation of Blakeslea Trispora
Foods, 2019 The production of lycopene from different substrates by Blakeslea trispora in fermentation was investigated. Lycopene productions from 4 and 6% glucose (pH 6.5) in shake flask fermentation were 77.7 and 28.1 mg L−1.
Ayse Sevgili, Osman Erkmen
doaj +1 more source
Identification of inhibitors of the Salmonella FraB deglycase, a drug target
FEBS Open Bio, EarlyView.A high‐throughput screen was used to identify inhibitors of Salmonella FraB, a drug target. Characterization of top hits (identified after an additional counter screen) revealed that some triazolidines, thiadiazolidines, and triazolothiadiazoles are mixed‐type inhibitors of FraB.
Jamison D. Law +6 more
wiley +1 more source
Acta Pharmaceutica, 2019 A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was ...
Brusač Edvin +3 more
doaj +1 more source
A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
Journal of Microbiology & Biology Education, 2023 Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size.
Lúcia Chaves Simões +4 more
doaj +1 more source
Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria
FEBS Open Bio, EarlyView.The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao +4 more
wiley +1 more source
Microbial Cell Factories, 2022 Background The fungal natural products illudin S and M have been investigated as precursors for the development of semisynthetic anticancer agents such as Irofulven (illudin S derivative) which is currently in phase II clinical trials.
Lillibeth Chaverra-Muñoz +1 more
doaj +1 more source