Results 51 to 60 of about 47,367 (336)

In situ recovery of secondary metabolites using adsorption resins : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy at Massey University, Palmerston North, New Zealand [PDF]

, 2006
Almost without exception a two to three fold increase in microbial secondary metabolite concentration was measured when adsorption resins were added in-situ during a submerged liquid fermentation. Anguidine was produced at a final concentration of 440 mg/
Ryan, Jason L. J
core  

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures [PDF]

, 2018
To increase the yield of heterologous production of the class II bacteriocin DvnRV41 with Escherichia coli Origami (DE3) (pLysS/pCR03), induction of bacteriocin gene expression was optimized by varying the inducer isopropyl β-d-thiogalactopyranoside ...
Calvez, Ségolène, Drider, Djamel, Konrad, Daniel, Lacroix, Christophe, Prévost, Hervé, Yildirim, Selcuk   +5 more
core  

Epigenetic and antitumor effects of platinum(IV)-octanoato conjugates [PDF]

, 2017
We present the anticancer properties of cis, cis, trans-[Pt(IV)(NH3)2Cl2(OA)2] [Pt(IV)diOA] (OA = octanoato), Pt(IV) derivative of cisplatin containing two OA units appended to the axial positions of a six-coordinate Pt(IV) center.
Brabec, Viktor, Gandin, Valentina, Gibson, Dan, Kasparkova, Jana, Marzano, Cristina, Novohradsky, Vojtech, Osella, Domenico, Pracharova, Jitka, Zanellato, Ilaria   +8 more
core   +1 more source

Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks

Biotechnology Journal
ABSTRACTShake flasks are predominantly used in screening and the early stages of biotechnological process development. However, oxygen‐demanding processes cannot easily be performed in shake flasks, since the maximum oxygen transfer capacity is usually smaller than in stirred reactors.
Schulte, Andreas, Jordan, Andreas, Klöckner, Wolf, Schumacher, Mathias, Corves, Burkhard, Büchs, Jochen   +5 more
openaire   +3 more sources

Combining antibody conjugates with cytotoxic and immune‐stimulating payloads maximizes anti‐cancer activity

Molecular Oncology, EarlyView.
Methods to improve antibody–drug conjugate (ADC) treatment durability in cancer therapy are needed. We utilized ADCs and immune‐stimulating antibody conjugates (ISACs), which are made from two non‐competitive antibodies, to enhance the entry of toxic payloads into cancer cells and deliver immunostimulatory agents into immune cells.
Tiexin Wang, Dong Jun Koo, Peter M. Tessier, Greg M. Thurber   +3 more
wiley   +1 more source

Citric acid production by immobilized cells of the yeast Candida guilliermondii : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology and Bioprocess Engineering at Massey University [PDF]

, 1995
The feasibility of using cells of Candida guilliermondii immobilized onto sawdust particles for production of citric acid was investigated. C. guilliermondii IMK1 from a stock culture (Department of Process and Environmental Technology, Massey University,
Tisnadjaja, Djadjat
core  

Novel methylotrophic bacteria isolated from the River Thames (London, UK) [PDF]

, 2008
Enrichment and elective culture for methylotrophs from sediment of the River Thames in central London yielded a diversity of pure cultures representing several genera of Gram-negative and Gram-positive bacteria, which were mainly of organisms not ...
Alves, Anesti, Anesti, Arfman, Bamforth, Barrow, Battistuzzi, Beji, Bergey, Bernardet, Bernardet, Borodina, Borodina, Boucher, Brautaset, Brocks, Buonaurio, Cawse, Cercel, Dedysh, Dedysh, Dijkhuizen, Dijkhuizen, Duménil, Frankland, Frankland, Fredericq, Galkin, Ghosh, Ginige, Green, Hartmans, Holt, Hormisch, Jakobsen, Kalyuzhnaya, Kanagawa, Kato, Kato, Kelley, Kelly, Kelly, Kodaka, Levering, Lidstrom, Lidstrom, Loew, McDonald, McDonald, Meganathan, Migula, Mitsui, Moosvi, Moosvi, Nazina, Nercessian, Nesvera, Nishio, Orita, Padden, Radajewski, Radajewski, Reizer, Schaeffer, Schäfer, Smith, Urakami, Wang, Ward, Ward, Warner, Wayne, Whittenbury, Yasueda, Yoon, Yurimoto   +75 more
core   +1 more source

Gram-scale production of recombinant microbial enzymes in shake flasks [PDF]

FEMS Microbiology Letters, 2017
Heterologous production of recombinant proteins is a cornerstone of microbiological and biochemical research as well as various biotechnological processes. Yields and quality of produced proteins have a tremendous impact on structural and enzymology studies, development of new biopharmaceuticals and establishing new biocatalytic processes.
Lukas, Chrast, Radka, Chaloupkova, Jiri, Damborsky   +2 more
openaire   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source