Results 51 to 60 of about 18,161 (258)

Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli

open access: yesBiotechnology & Biotechnological Equipment, 2021
2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to ...
Yingxue Liao   +6 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Improved Lycopene Production from Different Substrates by Mated Fermentation of Blakeslea Trispora

open access: yesFoods, 2019
The production of lycopene from different substrates by Blakeslea trispora in fermentation was investigated. Lycopene productions from 4 and 6% glucose (pH 6.5) in shake flask fermentation were 77.7 and 28.1 mg L−1.
Ayse Sevgili, Osman Erkmen
doaj   +1 more source

NanoMOF‐Based Multilevel Anti‐Counterfeiting by a Combination of Visible and Invisible Photoluminescence and Conductivity

open access: yesAdvanced Functional Materials, EarlyView.
This study presents novel anti‐counterfeiting tags with multilevel security features that utilize additional disguise features. They combine luminescent nanosized Ln‐MOFs with conductive polymers to multifunctional mixed‐matrix membranes and powder composites. The materials exhibit visible/NIR emission and matrix‐based conductivity even as black bodies.
Moritz Maxeiner   +9 more
wiley   +1 more source

Highly Sensitive Electrochemical Biosensor Based on Hairy Particles with Controllable High Enzyme Loading and Activity

open access: yesAdvanced Functional Materials, EarlyView.
For the first time, a highly sensitive electrochemical biosensor based on SiO2‐based hairy particles with a grafted PDMAEMA polymer brush containing a quantifiable and large amount of immobilized Laccase is reported. The fabricated biosensor exhibits a sensitivity of 0.14 A·m⁻¹, a limit of detection (LOD) of 0.1 µm, and a detection range of 0.3–750 µm,
Pavel Milkin   +7 more
wiley   +1 more source

A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks

open access: yesJournal of Microbiology & Biology Education, 2023
Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size.
Lúcia Chaves Simões   +4 more
doaj   +1 more source

Near‐Infrared Organic Photovoltaic Electrodes for Subretinal Neurostimulation

open access: yesAdvanced Functional Materials, EarlyView.
Organic photovoltaic electrodes based on the D18:Y6 blend enable precise and light‐controlled activation of retinal ganglion cells in a degenerating retina. NIR Light‐driven activation of retinal ganglion cells, tunable stimulation parameters, and biocompatibility with human retinal organoids highlight their potential for next‐generation prosthetics ...
Andrea Corna   +10 more
wiley   +1 more source

Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors

open access: yesMicrobial Cell Factories, 2022
Background The fungal natural products illudin S and M have been investigated as precursors for the development of semisynthetic anticancer agents such as Irofulven (illudin S derivative) which is currently in phase II clinical trials.
Lillibeth Chaverra-Muñoz   +1 more
doaj   +1 more source

Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast

open access: yesBiotechnology Reports, 2022
The commonly used host for industrial production of recombinant proteins Pichia pastoris, has been used in this work to produce the rabies virus glycoprotein (RABV-G). To allow a constitutive expression and the secretion of the expressed recombinant RABV-
Hana Askri   +3 more
doaj   +1 more source

A Smart Bio‐Battery Facilitates Diabetic Bone Defect Repair Via Inducing Macrophage Reprogramming and Synergistically Modulating Bone Remodeling Coupling

open access: yesAdvanced Functional Materials, EarlyView.
This research presents a novel implantable bio‐battery, GF‐OsG, tailored for diabetic bone repair. GF‐OsG generates microcurrents in high‐glucose conditions to enhance vascularization, shift macrophages to the M2 phenotype, and regulate immune responses.
Nanning Lv   +10 more
wiley   +1 more source

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