Identification of Shiga toxigenic Escherichia coli seropathotypes A and B by multiplex PCR
Molecular and Cellular Probes, 2007 A multiplex PCR assay was developed to identify the six clinically important enterohemorrhagic Escherichia coli (EHEC) serotypes classified in seropathotypes A and B and to differentiate these from Shiga toxigenic E. coli. The assay simultaneously detects genes for Shiga toxin (stx) and intimin (eae), including allelic variants of both genes, 16S ...
S.R. Monday, Arica Beisaw, Peter Feng
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Multiple evolutionary trajectories for non-O157 Shiga toxigenic Escherichia coli [PDF]
, 2019 AbstractBackgroundShiga toxigenic Escherichia coli (STEC) is an emerging global pathogen and remains a major cause of food-borne illness with more severe symptoms including hemorrhagic colitis and hemolytic-uremic syndrome. Since the characterization of the archetypal STEC serotype, E. coli O157:H7, more than 250 STEC serotypes have been defined.
Nicola K. Petty +13 more
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X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression [PDF]
J.Synchrotron Radiat. 20 (2013) 581-586, 2016 This paper reports X-ray microtomographic visualization of the microorganism Escherichia coli overexpressing a metalloprotein ferritin. The three-dimensional distribution of linear absorption coefficients determined using a synchrotron radiation microtomograph with a simple projection geometry revealed that the X-ray absorption was homogeneously ...
arxiv +1 more source
Molecular Surveillance of Shiga Toxigenic Escherichia coli O157 by PulseNet USA
Journal of Food Protection, 2005 PulseNet USA is the national molecular subtyping network system for foodborne disease surveillance. Sixty-four public health and food regulatory laboratories participate in PulseNet USA and routinely perform pulsed-field gel electrophoresis of Shiga toxigenic Escherichia coli isolated from humans, food, water, and the environment on a real-time basis ...
Marcus Head +12 more
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Detection of virulence genes in Shiga toxigenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic pediatric patients in Ondo State, Nigeria [PDF]
Microbes and Infectious Diseases, 2022 Background:Shiga toxigenic Escherichia coli (STEC) is one of the major causes of intestinal infection, bloody and non-bloody diarrhoea and extra-intestinal complications in humans.
Joy Olanrewaju, Anthony Onifade
doaj +1 more source
Genetic Diversity and Virulence Potential of Shiga Toxin-Producing Escherichia coli O113:H21 Strains Isolated from Clinical, Environmental, and Food Sources [PDF]
, 2014 Shiga toxin-producing Escherichia coli strains of serotype O113:H21 have caused severe human diseases, but they are unusual in that they do not produce adherence factors coded by the locus of enterocyte effacement.
Beutin, Lothar +9 more
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Presence of activatable Shiga toxin genotype (stx2d) in Shiga toxigenic Escherichia coli from livestock sources [PDF]
, 2003 Stx2d is a recently described Shiga toxin whose cytotoxicity is activated 10- to 1,000-fold by the elastase present in mouse or human intestinal mucus. We examined Shiga toxigenic Escherichia coli (STEC) strains isolated from food and livestock sources ...
Doi:j. Clin Microbiol +7 more
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Emerging Infectious Diseases, 2020 Signs and symptoms of Shiga toxin–producing Escherichia coli (STEC) serogroup O157:H7 infection range from mild gastrointestinal to bloody diarrhea and hemolytic uremic syndrome (HUS).
Lisa Byrne +2 more
doaj +1 more source
Supervivencia de VTEC O157 y no-O157 en agua de bebederos y materia fecal de bovinos [PDF]
, 2014 Verotoxin-producing E. coli (VTEC) are the etiologic agent of hemolytic-uremic syndrome (HUS), that affect children typically ranged in age from six months old to five years old.
Arroyo, Guillermo Horacio +3 more
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Pathologic changes in mice induced by subtilase cytotoxin, a potent new Escherichia coli AB(5) toxin that targets the endoplasmic reticulum [PDF]
, 2007 Subtilase cytotoxin (SubAB) is the prototype of a recently discovered AB₅ cytotoxin family produced by certain strains of Shiga toxigenic Escherichia coli (STEC).
Paton, A., Paton, J., Wang, H.
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